In this study, serotype-specific genes of Salmonella enterica serovar Derby (SD) (RU61_00441, RU61_00445, RU61_00447, RU61_RS09205, and RU61_RS06985) were identified by bioinformatics and comparative genomics analysis. Primers based on RU61_00441 and RU61_00447, as well as 139-141 primer were used to develop a multiplex polymerase chain reaction (PCR) assay. The assay was evaluated by its specificity, colony forming sensitivity, anti-interference ability, and artificial contamination. Positive results were showed with three specific bands: 171 bp, 284 bp, and 512 bp. There were 39 Salmonella strains and 18 non-Salmonella strains detected by this method, showing 100% specificity. The detection limits of this assay for DNA and colony forming were 383.2 pg/μL and 52 CFU/mL, respectively. When the background colony concentration ratio of SD to nature (pork, chicken, and beef) was 1∶104, three clear and accurate amplification bands were obtained. In artificially contaminated pork, chicken, and beef samples, this assay could detect as few as 3.8 CFU/25 g after 10 h enrichment. This method can detect SD accurately and sensitively, and therefore can be widely used for food safety.
ZHAI Ligong
,
YANG Jianting
,
LI Yongquan
,
WANG Shuiping
,
WANG Junying
. Screening serotype specific genes and developing a multiple PCR method to determine Salmonella enterica serovar Derby[J]. Food and Fermentation Industries, 2019
, 45(7)
: 269
-275
.
DOI: 10.13995/j.cnki.11-1802/ts.019243
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