A method using high performance chromatography tandem mass spectrometry/mass spectrometry(HPLC-MS/MS)detecting parvalbumin in Paralichthys olivaceus was established. ALTDAETK was used as a quantitative marker peptide, SDFIEEDELK and LFLQNFAFSASAR were used as qualitative marker peptides. The pretreatment conditions including protein extracts, indole-3-acetic acid (IAA) concentration, enzyme/protein ratio, and enzymatic hydrolysis time, as well as the instrumental conditions for mass spectrometry parameters used for three marker peptides were optimized. The results showed that ALTDAETK had a good linear relationship in a range of 0.005-10 mg/L (R2>0.999). The quantification limit of parvalbumin was 2.74 mg/kg. Took Psetta maxima as a blank, the average recovery rate of recombinant parvalbumin was 94.8%-102.0%, and the relative standard deviation (RSD) was below 7% with a good repeatability. It was the first time that parvalbumin was detected in Paralichthys olivaceus accurately and quantitatively using HPLC-MS/MS.
GE Minmin
,
WANG Jianhua
,
LIN Hong
. Determination of allergen parvalbumin in Paralichthys olivaceus using HPLC-MS/MS[J]. Food and Fermentation Industries, 2019
, 45(8)
: 210
-214
.
DOI: 10.13995/j.cnki.11-1802/ts.018474
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