A wild Amauroderma rugosum was collected from Ceheng, Guizhou. Its crude polysaccharides were extracted from liquid fermentation of isolated mycelium by hot water and ethanol precipitation. The crude polysaccharides were further purified by deproteinization, decoloration, and column chromatography, resulted refined polysaccharides (ARP). The structural characteristics of ARP were elucidated by chromatographic methods. Meanwhile, the antioxidant activities of crude and refined polysaccharides were compared. The results showed that the molecular weight of ARP was 1.86×104 Da. Moreover, IR and NMR chromatograms showed that they had characteristic peaks of polysaccharides. Its monosaccharide composition included rhamnose (Rha), mannose (Man), and galactose (Gal), with a molar ratio of 1∶1.24∶3.51. Furthermore, the mycelium polysaccharides demonstrated excellent antioxidant activities in vitro, and crude polysaccharides exhibited better antioxidative effects. When the concentration of polysaccharides was 1 mg/mL, the total reducing activity of crude polysaccharides was 1.72 times higher than that of the refined one. Both of these two polysaccharides were good at scavenging DPPH· (EC50: 0.05 and 1.15 mg/mL for crude and refined polysaccharides , respectively) and ABTS·(EC50∶0.15 and 1.49 mg/mL for crude and refined polysaccharides , respectively). This research provides scientific basis for exploiting and utilizing polysaccharides from A.rugosum.
WU Yan
,
SHEN Liqun
,
ZHU Hua
. Purification, structural characteristics, and in vitro antioxidant activities ofpolysaccharides ARP from a wild Amauroderma rugosum mycelium[J]. Food and Fermentation Industries, 2019
, 45(9)
: 214
-219
.
DOI: 10.13995/j.cnki.11-1802/ts.018120
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