This study aimed to investigate differential expression genes and changes in genes that are related to glutathione metabolism in a Hansenula anomala mutant, Ar_Han0458, during fermentation. The RNA-seq high-throughput sequencing data of Ar_Han0458 at five fermentation time points (0, 24, 48, 72, 96 h) were analyzed by bioinformatics methods. It was found that in comparison to 0 h, the number of down-regulated genes was more than that of up-regulated genes at each fermentation time. Except fermenting for 96 h and 72 h, the number of differential expression genes at adjacent time points was mainly down-regulated. Besides, the gene expression profiles were similar between 48 and 72 h. Moreover, the GO functions of differentially expressed genes at various time points mainly involved in cell process, cells and catalytic activity. The results of KEGG enrichment indicated that 10 differentially expressed genes participated in glutathione metabolism. The synthesis rate of glutathione reached the highest in 24 and 96 h, which was consistent with the variation trend of extracellular glutathione production. In conclusion, the number of differentially expressed genes in Ar_Han0458 was mainly up-regulated and it had the highest glutathione synthesis rate at 96 h. This study provides a theoretical basis for metabolic regulation of recombinant H. anomala and its molecular breeding.
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