Isomaltooligosaccharides (IMOs) are a kind of functional oligosaccharides that have been widely used in food industries. A new procedure for IMOs preparation was developed by using whole-cell of Aspergillus niger H9-30 as a catalyst and maltose as substrate. The optimal reaction condition in shaking flasks was as follows: at 48 ℃ with an initial pH of 4.2, with 600 g/L maltose and 15 g/L A. niger H9-30. Under this condition, the yields of isomaltose, panose, and isomaltotriose accounted for 50.5% of total sugar, and total IMOs content accounted for 63.3%. It was also found that using whole-cell of A. niger to produce IMOs had good operational stability with the half-life reached 20 batches (10 d). In conclusion, A. niger H9-30 has potential use for industrial production of IMO.
HUANG Nan
,
ZHOU Bo
,
YE Tong
,
CHEN Guiguang
,
LIANG Zhiqun
,
ZENG Wei
. Synthesis of isomaltooligosaccharides by whole-cell Aspergillus niger H9-30[J]. Food and Fermentation Industries, 2019
, 45(10)
: 36
-41
.
DOI: 10.13995/j.cnki.11-1802/ts.019608
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