Effects of T-2 toxin on in vitro proliferation and apoptosis of human renal tubular epithelial cell HK-2

  • XIAN Tingting ,
  • HAN Xiaomin ,
  • HAN Chunhui ,
  • LI Fengqin ,
  • XU Wenjing ,
  • ZHANG Hongyuan ,
  • TU Kang
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  • 1(College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)
    2(NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100021,China)

Received date: 2018-12-21

  Online published: 2019-06-17

Abstract

This study aimed to study the effects of T-2 toxin on proliferation and apoptosis of human renal tubular epithelial cell HK-2. The HK-2 cells were treated with different concentrations of T-2 toxin for 72 h, followed by measuring their proliferation inhibition rate, cell morphology, DNA fragmentation, apoptosis rate, cell cycle distribution, and caspase-3 activity. The results showed that T-2 toxin inhibited HK-2 cell proliferation and the inhibition rate increased with increasing T-2 toxin concentration (IC50 =49.34 nmol/L). Moreover, T-2 toxin induced cell apoptosis of HK-2 cells in a positive dose-dependent manner in the range of 10-40 nmol/L. Additionally, 20 nmol/L T-2 toxin resulted in chromatin condensation and other typical characteristics of apoptotic cells. Furthermore, 10-40 nmol/L T-2 toxin affected the cell cycle distribution by arresting the G2/M phase and dose-dependently increased the caspase-3 activity with maximally 1.93-fold higher than the control (P<0.05). This paper provides a theoretical basis to elucidate the mechanisms of adverse effects of T-2 toxin on the kidneys.

Cite this article

XIAN Tingting , HAN Xiaomin , HAN Chunhui , LI Fengqin , XU Wenjing , ZHANG Hongyuan , TU Kang . Effects of T-2 toxin on in vitro proliferation and apoptosis of human renal tubular epithelial cell HK-2[J]. Food and Fermentation Industries, 2019 , 45(10) : 86 -92 . DOI: 10.13995/j.cnki.11-1802/ts.019705

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