This study aimed to study the effects of T-2 toxin on proliferation and apoptosis of human renal tubular epithelial cell HK-2. The HK-2 cells were treated with different concentrations of T-2 toxin for 72 h, followed by measuring their proliferation inhibition rate, cell morphology, DNA fragmentation, apoptosis rate, cell cycle distribution, and caspase-3 activity. The results showed that T-2 toxin inhibited HK-2 cell proliferation and the inhibition rate increased with increasing T-2 toxin concentration (IC50 =49.34 nmol/L). Moreover, T-2 toxin induced cell apoptosis of HK-2 cells in a positive dose-dependent manner in the range of 10-40 nmol/L. Additionally, 20 nmol/L T-2 toxin resulted in chromatin condensation and other typical characteristics of apoptotic cells. Furthermore, 10-40 nmol/L T-2 toxin affected the cell cycle distribution by arresting the G2/M phase and dose-dependently increased the caspase-3 activity with maximally 1.93-fold higher than the control (P<0.05). This paper provides a theoretical basis to elucidate the mechanisms of adverse effects of T-2 toxin on the kidneys.
XIAN Tingting
,
HAN Xiaomin
,
HAN Chunhui
,
LI Fengqin
,
XU Wenjing
,
ZHANG Hongyuan
,
TU Kang
. Effects of T-2 toxin on in vitro proliferation and apoptosis of human renal tubular epithelial cell HK-2[J]. Food and Fermentation Industries, 2019
, 45(10)
: 86
-92
.
DOI: 10.13995/j.cnki.11-1802/ts.019705
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