The accumulation is a key process in the production of sesame flavor liquor, and directly affects the subsequent fermentation and the quality of the final sample. Effected by the temperature and oxygen concentration in the accumulation process, at the end of the accumulation, there are significant differences in the amount of yeast and bacteria between the surface and the core, and the saccharification enzymes, starch, acidity, reducing sugar and alcohol between them were also different. In order to investigate the effect of two different accumulation grains on the production of sesame flavor liquor, the actual temperature of fermentation in the pit was simulated by laboratory temperature control, and the surface grains and the core grains were mixed according to different mixing ratio for fermentation. The dynamic changes of physical and chemical indexes in the fermentation process were studied and the final samples were analyzed by GC-MS combined with sensory evaluation. The results showed that there are differences in the physical and chemical indexes of different mixing ratio. The actual production of mixed fermented grains was the closest to the original wine samples of the factory. The middle grains in a larger proportion produced the fermented wine with sesame flavor, it was speculated that the formation of the core grains and the sesame flavor liquor had a great relationship.