This study aimed to develop a rapid and simple pH-sensitive colorimetric system based on poly-(γ-glutamic acid)-modified gold nanoparticles (PGA-AuNPs). Electrostatic self-assembly technique was used to assemble negatively charged PGA to the surface of positively charged cetyltrimethylammonium bromide (CTAB)-reduced star like AuNPs. The physicochemical properties of PGA-AuNPs were investigated, and its sensitivity to pH was evaluated by both colorimetry and fluorescence spectrometry. The results showed that the mean diameter of PGA-AuNPs was (28.93±8.96) nm with 21 mV surface potential. The color of PGA-AuNPs changed from purple to blue and fluorescence intensity gradually weakened at pH 5 to 12, which was higher than its isoelectric point (pKa=4-5). At pH lower than its pKa, its color changed from purple to red and eventually to colorless, along with gradual decline in the fluorescence intensity. In conclusion, the pH-sensitive PGA-AuNPs indicates its potential applications in food processing, bioanalysis and drug delivery.
GUAN Huanan
,
GONG Dezhuang
,
SONG Yan
,
HAN Bolin
,
LIANG Jinzhong
,
WANG Wei
,
YU Jia
. Poly-(γ-glutamic acid) modified star-like gold nanoparticles and their pH sensitivity[J]. Food and Fermentation Industries, 2019
, 45(12)
: 41
-45
.
DOI: 10.13995/j.cnki.11-1802/ts.019770
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