In order to reduce the death rate of Bifidobacterium during industrial preparation, its death patterns at different stages before freeze-drying were experimentally analyzed. The survival rates of Bifidobacterium bifidum CCFM16, Bifidobacterium adolescentis CCFM8630, Bifidobacterium brevis CCFM683 and Bifidobacterium longum L6 were determined under different processing conditions. It was found that the survival rate of the four strains decreased about 20% after exposing the fermentation broth to air for 3 h. Quick centrifugation at 4 °C was beneficial for lowering the death rate, while centrifugation force in a certain range had no significant effect. Survival rate of the pellet decreased about 10% after exposing to air for 1 h. The same decrease was found while the mixture of lyoprotectant and bacterial suspension exposed to air for 2 h, and its survival rate decreased about 20% in a 3 h exposure. Additionally, their survival rates were significantly improved by D-isoascorbate. This study clarified the key factors that influence the survival rate of Bifidobacterium during preparation and therefore has guiding significance for improving its production efficiency.
WANG Shunyu
,
ZHANG Hairong
,
LI Yuan
,
HE Jianxin
,
LU Yuanyuan
,
CUI Shumao
,
LU Wenwei
,
ZHANG Hao
. Simulation analysis of death patterns of Bifidobacterium at different stages before freeze-drying at industrial scale[J]. Food and Fermentation Industries, 2019
, 45(12)
: 55
-61
.
DOI: 10.13995/j.cnki.11-1802/ts.019259
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