In order to further increase mannitol production from sucrose in Leuconostoc sp., a mt1d-m1p expression cassette was site-directly integrated onto its chromosome. The resulting strains, Δaldh::(mt1d-m1p), Δaldh::(mt1d-m1p) Δdts::amy, Δaldh::(mt1d-m1p) Δdts::(mt1d-m1p), and Δdts1 ΔD-ldh Δpat::mdh Δstpk::mdh Δfk::mdh Δaldh::(mt1d-m1p) produced 42.63, 43.47, 45.74 and 47.26 g/L mannitol from 90 g/L sucrose. In comparison, wild type only produced 31.48 g/L mannitol. In conclusion, increasing the synthetic route of mannitol from glucose is one of the means to increase its production.
JIN Hongxing
,
WANG Xing
,
PENG Yuwei
. Enhanced biosynthesis of mannitol in Leuconostoc sp. expressed mt1d-m1p[J]. Food and Fermentation Industries, 2019
, 45(12)
: 96
-100
.
DOI: 10.13995/j.cnki.11-1802/ts.019858
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