This study was conducted to study differences in γ-aminobutyric acid (GABA) production in Lactobacillus and the correlation between its yield and key genes expressions in the glutamate decarboxylase (GAD) system. The results showed that most GABA-producing strains belonged to L. brevis and L. plantarum. The transcriptional activities of GAD-related genes at different growth stages of L. brevis and L. plantarum were studied by qRT-PCR. It was found that the expression levels of gadB and gadC in L. brevis were similar and peaked at late logarithm or early stable phase. Therefore, the high-yield of GABA in L. brevis may be associated with high expressions of gadC and gadB. Moreover, the expression of gadB in L. plantarum was similar to that of L. brevis, but its GABA production had little correlation with gadB expression, which indicated that other factors might affect L. plantarum producing GABA. Overall, this study provides a theoretical basis for screening GABA-producing strains and regulating GAD gene expression in Lactobacillus as well as for optimizing GABA fermentation process.
HAN Xiao
,
YU Leilei
,
ZHAI Qixiao
,
CUI Shumao
,
ZHAO Jianxin
,
ZHANG Hao
,
TIAN Fengwei
,
CHEN Wei
. Production of γ-aminobutyric acid by Lactobacillus brevis and Lactobacillus plantarum and key relevant genes analysis[J]. Food and Fermentation Industries, 2019
, 45(13)
: 1
-8
.
DOI: 10.13995/j.cnki.11-1802/ts.020342
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