The surfactin producing strains were screened directionally using oil repellency, emulsifying ability and surface tension of the fermentation supernatant surfactant as preliminary screening parameters, and srfA gene for rescreening. A surfactin high-yield strain 41-3 utilizing brewer's spent grain (BSG) was screened by thin layer chromatography (TLC) qualitative analysis and ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight high-resolution/mass spectrometry (UPLC-ESI/Q-TOF MS) quantitative analysis. The strain was identified as Bacillus subtilis, and different congeners of surfactin were analyzed. The surfactin yield of B. subtilis 41-3 was 541.32 mg/L in MSM medium, and C14 and C13 fatty-acid-chain surfactins were the two most abundant homologues, which accounted for 75% of total surfactin. When BSG was used as fermentation substrate, the yield of surfactin increased 1.38 times to 746.35 mg/L, and the surfactin synthesis shifted to long fatty-acid-chain surfactins, which indicated better biosurface activity. This study provides new ideas for surfactin-producing strain screening and new raw materials for surfactin production, as well as revealing a new way for comprehensive utilization of BSG.
LI Yanlin
,
ZHANG Wei
,
LI Xiaoyu
,
LU Xinyi
,
CAO Yu
. Screening and characteristics of surfactin-producing Bacillus[J]. Food and Fermentation Industries, 2019
, 45(13)
: 31
-37
.
DOI: 10.13995/j.cnki.11-1802/ts.019918
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