The characteristics of cholesterol esterase derived from Burkholderia cepacia ZWS15 were systematically studied. The purified cholesterol esterase was obtained by DEAE ion exchange chromatography from culture supernatant and its tolerance to organic solvents and detergents, as well as its hydrolysis characteristics with different substrates were determined. The enzyme was stable at pH 5.5-9.0 and showed optimal activity at 40 ℃. More than 70% of enzyme activity could be retained after 2 h incubation at 70 ℃, indicated it was remarkably resistant to heat. Moreover, relatively high enzyme activity could be obtained even in the presence of 50% organic solvents and 5% detergents. Its molecular weight was revealed as 37 kDa by time-of-flight mass spectrometry and high matching degrees were obtained with cholesterol esterase (source of Burkholderia cepacia) and lipase (source of Pseudomonas sp.). Compared to commercially available cholesterol esterase, the enzyme not only preferentially hydrolyzed long-chain fatty acid esters of cholesterol, but also exhibited lipolytic activity towards various triacylglycerides and p-nitrophenyl esters. Overall, this study provides a reference for applying cholesterol esterase in foods, sewage treatment, pulping and papermaking industries.
SUN Liuqing
,
WU Mengqi
,
ZHANG Ling
,
WU Di
,
XIN Yu
,
YANG Hailin
. Characterization of cholesterol esterase with lipolytic activity from Burkholderia cepacia[J]. Food and Fermentation Industries, 2019
, 45(14)
: 9
-14
.
DOI: 10.13995/j.cnki.11-1802/ts.020089
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