In order to obtain a recombinant pullulanase suitable for vermicelli production, the pullulanase encoding gene GsP from Geobacillus stearothermophilus was secretorily expressed in Bacillus subtilis 1A717 and WB600. The recombinant pullulanase expressed in B. subtilis 1A717 and B. subtilis WB600 were GsP1a and GsPwb, respectively. Cooking experiments showed that the broken rate of vermicelli made from GsP1a-treated paste was almost 0, while the broken rate of vermicelli made from GsPwb-treated paste was notably higher due to starch degradation by α-amylase produced by host strain WB600. This study shows that neutrophilic pullulanase is an ideal alum substitute in vermicelli production, and inactivating α-amylase is beneficial to improve the quality of the product.
WANG Jie
,
ZHU Xinwen
,
Deqingmeiduo
,
YAO Yan
,
SHEN Wei
,
CHEN Xianzhong
,
FAN You
. High expression of a neutrophilic pullulanase and its application in vermicelli production[J]. Food and Fermentation Industries, 2019
, 45(14)
: 122
-127
.
DOI: 10.13995/j.cnki.11-1802/ts.020066
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