Food and Fermentation Industries >

2019 , Vol. 45 >Issue 14: 233 - 238

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.019291

Application of recombinase-polymerase mediated isothermal amplification in food safety analysis

  • LAN Haiou ,
  • KE Yiqiang ,
  • MA Xianying ,
  • CHENG Hao ,
  • DING Gongtao ,
  • LI Mingsheng ,
  • CHEN Shien ,
  • MA Zhongren ,
  • WEI Jia
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  • 1 China-Malaysia National Joint Laboratory, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China
    2College of Life Sciences and Engineering,Northwest Minzu University, Lanzhou 730030, China

Received date: 2018-11-12

  Online published: 2019-08-20

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Abstract

Recombinase-polymerase amplification (RPA) is a new isothermal amplification technique for detecting specific nucleotide sequences. Compared with regular PCR amplification, it has advantages of high sensitivity, high specificity, short detection time and simple operation. At optimal temperature (37-42℃), RPA can be used to amplify specific nucleotide sequences rapidly with simple equipment even in harsh environment. The detection results can be qualitatively and quantitatively analyzed by combining with fluorescence detection device of side-flow chromatography technology. The principles and operating conditions of RPA as well as its applications in detecting food-borne virus, pathogenic bacteria, animal-derived components and transgenic foods were summarized in this paper in order to provide a reference for further extensive researches on RPA.

Key words： recombinase polymerase amplification; food safety; detection technology

Cite this article

LAN Haiou , KE Yiqiang , MA Xianying , CHENG Hao , DING Gongtao , LI Mingsheng , CHEN Shien , MA Zhongren , WEI Jia . Application of recombinase-polymerase mediated isothermal amplification in food safety analysis[J]. Food and Fermentation Industries, 2019 , 45(14) : 233 -238 . DOI: 10.13995/j.cnki.11-1802/ts.019291

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