Food and Fermentation Industries >

2019 , Vol. 45 >Issue 15: 279 - 287

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.020130

Quantitative detection of meat adulteration by real-time fluorescent PCR

  • PENG Yuanyuan ,
  • WU Xuan ,
  • TAO Xiaoqi
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  • 1College of Food Science, Southwest University, Chongqing 400715, China
    2National Food Science and Engineering Experimental Teaching Center Southwest University, Chongqing 400715, China
    3Chongqing Animal Disease Prevention and Control Center, Chongqing 401120, China

Online published: 2019-09-03

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Abstract

Meat adulteration has seriously threatened the public interest, therefore, establishing a fast and accurate detection method for meat identification has gradually risen public concern. PCR-based real-time fluorescent PCR can be used for qualitative and quantitative analysis and has the characteristics of higher automation degree and dynamic range. Therefore, it has great application potential for detecting meat adulteration. Based on the principle of real-time fluorescent PCR, this review further explored its operation process and integrated different types of real-time fluorescent PCR used for meat adulteration detection. For meat identification, real-time fluorescent PCR technology can use either fluorescent probe or fluorescent dye. These technologies still have some challenges in the process of continuous development and improvement in recent years to increase efficiency and accuracy. Overall, this review proposed to establish a standardized inspection process based on existing research and new technologies in order to further develop meat adulteration detection technology.

Key words： real-time PCR; meat adulteration; quantitative analysis

Cite this article

PENG Yuanyuan , WU Xuan , TAO Xiaoqi . Quantitative detection of meat adulteration by real-time fluorescent PCR[J]. Food and Fermentation Industries, 2019 , 45(15) : 279 -287 . DOI: 10.13995/j.cnki.11-1802/ts.020130

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