In order to enhance the efficiency of bacitracin fed-batch fermentation, effects of pH coupling, intermittent feeding-tank pressure control, intermittent-dissolved oxygen (DO) coupling and other feeding strategies on bacitracin synthesis were investigated in a 50 L fermenter. The amount of bacitracin produced by pH coupling strategy was 1 006 U/mL. However, in middle and late stages of logarithmic growth, glucose could not be filled in due to pH below the set value. Although bacitracin produced by intermittent feeding-tank pressure control strategy was 11% higher than that of pH coupling strategy, it also had a problem of sugar-DO mismatched at late fermentation stage. The intermittent-DO coupling strategy overcame the above drawbacks, the conversion rate of sugar to bacitracin (YP/S) increased by 22.7% compared with that of intermittent feeding-tank pressure control strategy. Based on intermittent-DO coupling feeding strategy and secondary nitrogen source feeding at 24 h, the average bacitracin synthesis rate reached 40.67 U/(mL·h), and the peak value of bacitracin production reached 1 220 U/mL. Therefore, a feeding strategy based on comprehensive carbon-nitrogen-oxygen factor of cell demand is established, which provides an important reference for industrialized fed-batch fermentation to produce bacitracin.
YANG Hua
,
SONG Zhao
,
DAI Hang
,
CHEN Xiong
,
LI Xin
,
CHEN Shouwen
,
CAI Dongbo
,
LI Junhui
,
WANG Zhi
. Feeding batch fermentation strategy based on cellular demands of carbon-nitrogen-oxygen promotes bacitracin synthesis[J]. Food and Fermentation Industries, 2019
, 45(15)
: 30
-36
.
DOI: 10.13995/j.cnki.11-1802/ts.020397
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