The surface-enhanced raman scattering (SERS) technique was used to detect melamine in milk. The metal titanium plate was used as a SERS substrate material, 50 nm silver nanoparticles were substrates, the ratio of the silver nanoparticles to the sample was 1∶2, and concentrations of both NaCl and NaOH were 4 mol/L. The SERS signal was acquired by a portable raman spectrometer. It was found that the intensity of SERS increased as the concentration of melamine increased from 0.2 mg/L to 10 mg/L (R2=0.998). Moreover, the detection limit was 0.08 mg/L and the relative standard deviation of the characteristic peak intensity of melamine was 4.34%. The established method was simple and easy to perform with good reproducibility and high stability. It can realize rapid detection of melamine in milk, which provides a reference for applying SERS in detecting pollutants in foods.
FENG Yanting
,
LIN Peichun
,
XIE Huifeng
,
WANG Ling
,
LI Chengyong
. Determination of melamine in milk based on silver nanoparticles aggregation by surface enhanced raman spectroscopy[J]. Food and Fermentation Industries, 2019
, 45(15)
: 256
-261
.
DOI: 10.13995/j.cnki.11-1802/ts.019596
[1] 邵静君,温家琪,徐世文.三聚氰胺毒理学研究进展[J].现代畜牧兽医,2007 (12):52-54.
[2] MENBERE L M, SU W N, CHEN C H, et al. Ag@SiO2 nanocubes loaded miniaturized filter paper as hybrid flexible plasmonic SERS substrate for trace melamine detection[J].Analytical Methods,2017(9):6 823-6 829.
[3] LIANG Xiu, ZHANG Xiaojuan, YOU Tingting, et al. Three-dimensional MoS2-NS@Au-NPs hybrids as SERS sensor for quantitative and ultrasensitive detection of melamine in milk[J].Journal of Raman Spectroscopy,2017(6):1-11.
[4] LIN Mengshi. A review of traditional and novel detection techniques for melamine and its analogues in foods and animal feed[J].Frontiers of Chemical Engineering in China,2009,3(4):427.
[5] XUE Yanpeng, SCAGLIONE F, RIZZI P, et al. High performance SERS on nanoporous gold substrates synthesized by chemical de-alloying a Au-based metallic glass[J].Applied Surface Science,2017,426:1 113-1 120.
[6] LIU L X, WU F F, XU D, et al. Space confined electroless deposition of silver nanoparticles for highly-uniform SERS detection[J].Sensors & Actuators B Chemical,2017,255:1 401-1 406.
[7] 徐昊,刘庆军,马蓉.含乳食品中三聚氰胺检测方法研究进展[J].绿色科技,2015(8):265-267.
[8] 徐晨曦,付大友,汪菊.乳制品中三聚氰胺检测方法的研究进展[J].现代化工,2014,34(3):154-157.
[9] 杨欣,王军,苗虹,等.牛奶中三聚氰胺的表面增强拉曼光谱检测法与国标检测法的比较[J].食品安全质量检测学报,2014,5(10):3 219-3 222.
[10] 胡玉兰,黄亚伟,王若兰,等.便携式拉曼光谱仪在食品检测中的应用[J].食品工业科技,2017,38(17):319-323.
[11] 刘宸,黄文倩,王庆艳,等.拉曼光谱在食品无损检测中的应用[J].食品安全质量检测学报,2015,6(8):2 981-2 987.
[12] 雷皓宇,陈小曼,李攻科,等.表面增强拉曼光谱法同时检测奶粉中三聚氰胺和二聚氰胺[J].分析科学学报,2017,33(3):312-316.
[13] 刘安琪,李攻科,胡玉玲.表面增强拉曼光谱快速检测食品添加剂的研究进展[J].食品安全质量检测学报,2015(6):2 214-2 223.
[14] ZEMAN E J, SCHATZ G C. An accurate electromagnetic theory study of surface enhancement factors for silver, gold, copper, lithium, sodium, aluminum, gallium, indium, zinc, and cadmium[J].Journal of Physical Chemistry,1987,91(3):634-643.
[15] 张孝芳,邹明强,陈彦长,等.用于液态奶中三聚氰胺现场快速检测的拉曼光谱法及试剂盒:中国,101477051B[P].2010.
[16] 赵蕊池,王培龙,石雷,等.自组装有序纳米银线表面增强拉曼光谱检测牛奶中三聚氰胺[J].分析化学,2017,45(1):75-82.
[17] LEE P C, MEISEL D. Adsorption and surface-enhanced raman of dyes on silver and gold sols[J].Journal of Physical Chemistry,1982,86(17):3 391-3 395.
[18] 李青青.基于聚甲基丙烯酸酯多孔材料的表面增强拉曼光谱检测方法研究[D].上海:华东理工大学,2013.
[19] 司民真.纳米银和纳米银膜制备及表面增强拉曼效应研究[D].哈尔滨:哈尔滨工业大学,2010.
[20] 林翔.SERS基底的制备及其用于食品中污染物的快速检测[D].哈尔滨:哈尔滨工业大学,2016.
[21] HOU Xu, WANG Qi, MAO Guoming, et al. Periodic silver nanocluster arrays over large-area silica nanosphere template as highly sensitive SERS substrate[J].Applied Surface Science,2018,437:92-97.
[22] XIE Yunfei, ZHU Xinyu, SUN Yingying, et al. Rapid detection method for nitrofuran antibiotic residues by surface-enhanced Raman spectroscopy[J].European Food Research & Technology,2012,235(3):555-561.
[23] LIN Xiang, HASI Wuliji, LOU Xiutao, et al. Droplet detection: simplification and optimization of detecting conditions towards high sensitivity quantitative determination of melamine in milk without any pretreatment[J].RSC Advances,2014,4(93):51 315-51 320.
[24] ZHANG Xiaofang, ZOU Mingqiang, QI Xiaohua, et al. Detection of melamine in liquid milk using surface-enhanced Raman scattering spectroscopy[J].Journal of Raman Spectroscopy,2010,41(12):1 655-1 660.
[25] 中华人民共和国卫生部,中华人民共和国工业和信息化部,中华人民共和国农业部等.卫生部等5部门关于三聚氰胺在食品中的限量值的公告:卫生部发[2011].10号[EB/OL].(2011-04-07)[2019-06-28].http://www.gov.cn/fwxx/jk/2011-04/20/content_1848408.htm.
