Food and Fermentation Industries >

2019 , Vol. 45 >Issue 21: 38 - 45

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.021589

Screening of Lactobacillus plantarum with higher yield of exopolysaccharides andoptimization of fermentation conditions

  • ZHANG Wenping ,
  • ZHAO Yingjie ,
  • LUO Sheng ,
  • CHENG Xin
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  • College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2019-07-07

  Online published: 2019-11-15

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Abstract

In order to obtain the lactic acid bacteria (LAB) with high exopolysaccharide (EPS)-producing ability, a strain of LPC-1 was finally screened by observing whether they could form sticky colonies and sulfuric acid-phenol method, which was identified as Lactobacillus plantarum. Using the yield of extracellular polysaccharide as an indicator, the fermentation process was optimized by single factor and response surface tests. The results showed that the optimal culture conditions were: 30 g/L sucrose and 10 g/L soybean peptone, inoculation amount of 4%, fermented at 30 ℃ and pH 6.5 for 24 h. The production of EPS was significantly affected by temperature, pH and concentration of diammonium citrate as determined by the Plackett-Burman test. Combined with the central combination test and response surface analysis, the optimal fermentation conditions were 32 ℃, pH 6.7 and 3 g/L diammonium citrate. Under this condition, the EPS yield was 2 064.69 mg/L, which was consistent with the predicted value and increased by 48.64% compared with that before optimization. These results provided a basis for the scale production of EPS by LAB.

Key words： Lactobacillus plantarum; exopolysaccharides; optimization; Plackett-Burman design; response surface methodology

Cite this article

ZHANG Wenping , ZHAO Yingjie , LUO Sheng , CHENG Xin . Screening of Lactobacillus plantarum with higher yield of exopolysaccharides andoptimization of fermentation conditions[J]. Food and Fermentation Industries, 2019 , 45(21) : 38 -45 . DOI: 10.13995/j.cnki.11-1802/ts.021589

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