The effects of phosphate-β-galactosidase and β-galactosidase on lactose metabolism in Lactococcus lactis (L. lactis) were investigated. The growth, acid production, lactose metabolism, activities of β-galactosidase and phosphate-β-galactosidase of 12 strains were determined in MRS-lactose broth where lactose was the sole carbon source. In order to understand the differences between these two galactosidases, the galactose accumulation in the fermentation broth was determined. The results showed that the lactose metabolism of L. lactis had significant differences. Moreover, the galactose contents in the fermentation broth of strains with high β-galactosidase activities ranged from 270 mg/L to 4110 mg/L, while the galactose contents of strains with high phosphate-β-galactosidase activities were in the range of 40 mg/L to 900 mg/L. Combining L. lactis, which had high phosphate-β-galactosidase activities, with Streptococcus thermophilus could reduce the content of galactose. The experimental results showed that L. lactis with high phosphate-β-galactosidase activities contributed to the reduction of the galactose content in fermented dairy products.
DENG Xiaoying
,
YANG Yu
,
JIANG Yang
,
LIU Xiaoming
,
ZHAO Jianxin
,
ZHANG Hao
,
CHEN Wei
. Galactosidases biodiversity in lactose metabolism among Lactococcus lacits[J]. Food and Fermentation Industries, 2019
, 45(21)
: 8
-14
.
DOI: 10.13995/j.cnki.11-1802/ts.020100
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