Proteins that interacted with the regulatory subunit of acetohydroxy acid synthase (IlvN) in Corynebacterium glutamicum were studied by his-pull down technique and liquid chromatography-mass spectrometry. Totally, 45 proteins were identified and most of them were ribosomal proteins involved in translation. The proteins involved in energy production and conversion, signal transduction , as well as transport and metabolism of amino acids, carbohydrates and lipids were also included. As acetohydroxy acid synthase is a key enzyme in branched-chain amino acid synthesis, the identification of its interacting proteins has great significance for further coordinate metabolic engineering.
HOU Zhengjie
,
ZHANG Quanwei
,
MO Xiaolin
,
XIA Li
,
TAN Miao
,
SUN Quanwei
,
MA Qian
,
CHEN Ning
. Identification of protein interaction with acetohydroxy acid synthase IlvN in Corynebacterium glutamicum by his-pull down combined with mass spectrometry[J]. Food and Fermentation Industries, 2019
, 45(16)
: 11
-17
.
DOI: 10.13995/j.cnki.11-1802/ts.020456
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