In order to enhance the freshness of yeast extract to better meet its needs in food industries, the effects of sodium citrate-molasses feeding strategy on the growth and intracellular glutamate synthesis in Saccharomyces cerevisiae J-5 were studied at a 10 L fermenter level. During molasses-fed fermentation, there was a significant overflow metabolism, and the ethanol concentration reached 22 g/L in 10 h. Besides, the dry weight and intracellular glutamate reached 36.36 g/L in 21 h and 2.28% in 18 h, respectively. Adding 1.8 L molasses that contained 72 g sodium citrate from 3 h to 14 h at an initial rate of 50 mL/h with an increasing flow rate of 50 mL every 2 h, the carbon overflow metabolism was effectively reduced. Moreover, the ethanol concentration in 10 h was only 54.5% of the control. Furthermore, the intracellular glutamate synthesis efficiency significantly improved, and the peak content reached 3.7% in 11 h, which was 62.3% higher than that of the control. In addition, the parameter of citrate content/the maximal cell dry weight under the optimized condition was 0.238±0.007, which provides a reference for industrial scale-up experiments.
TANG Chao
,
LIU Licong
,
CHEN Xiong
,
DAI Jun
,
LI Xin
,
YAO Juan
,
ZHENG Guobin
,
WANG Zhi
. Citrate enhanced intracellular glutamate production in Saccharomyces cerevisiae[J]. Food and Fermentation Industries, 2019
, 45(16)
: 48
-53
.
DOI: 10.13995/j.cnki.11-1802/ts.020402
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