In order to save the amount of steam used in fermentation broth sterilization, ozone sterilized water was used to replace part of the water in polysaccharide fermentation broth and the influences on fermentation process and results were studied. The results showed that 3, 4 and 5 mg/L ozone could sterilize tap water for fermentation in 120, 60, and 40 min, respectively. With 50% water replaced by ozone-sterilized tap water in the fermentation broth, there were no changes in reducing sugar and polysaccharides during or at the end of the fermentation, both in shake flask and pilot-scale fermentation, compared with steam-sterilized fermentation broth. Moreover, it was found that ozone-sterilized fermentation broth could save 47.3% steam and reduce 5 835 kg cooling water in a 10 m3 fermentor. Therefore, using ozone sterilized water can save a large amount of steam and cooling water, which provides a new method for energy-saving in fermentation industry.
WANG Shouquan
,
YU Zongyi
,
YUAN Dongling
,
CHAI Benyin
,
YIN Fengjiao
,
SHI Yongchun
. Industrial application of ozone for sterilizing fermentation broth and the energy-saving calculation[J]. Food and Fermentation Industries, 2019
, 45(16)
: 228
-232
.
DOI: 10.13995/j.cnki.11-1802/ts.020468
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