Analysis of polysaccharides and polypeptides in cereal vinegar sediment

  • GONG Tingting ,
  • LU Zhenming ,
  • GENG Yan ,
  • LONG Rong ,
  • WANG Haixiang ,
  • SHI Xiaohe ,
  • SHI Jinsong ,
  • XU Zhenghong
Expand
  • 1 (Jiangnan University, School of Pharmaceutical Science, Wuxi 214122, China)
    2 (National Engineering Laboratory for Cereal Fermentation Technology (Jiangnan University), Wuxi 214122, China)
    3 (Jiangnan University, School of Biotechnology, Wuxi 214122, China)
    4 (Hunan Weilai Yaoyuan Pharma Co. Ltd, Changsha 410013, China)

Received date: 2019-03-13

  Online published: 2019-09-23

Abstract

The contents and compositions of polysaccharides and polypeptides in cereal vinegar sediment and vinegar ethanol precipitate were analyzed. The polysaccharides content, the sulfate group content and monosaccharide composition of polysaccharides, and the polypeptides content and hydrolyzed amino acids compositions were determined. The relative contents of polypeptides and polysaccharides found to be 67.4 g/100 g and 73.0 g/100 g in the vinegar sediment and vinegar ethanol precipitate, respectively. There were six kinds of monosaccharides in crude polysaccharides of vinegar sediment and vinegar ethanol precipitate. The relative content of glucosamine (27.4%) in the polysaccharides from vinegar sediment was significantly higher than that from vinegar ethanol precipitate (3.2%). The sulfate group content of crude polysaccharides in vinegar sediment and vinegar ethanol precipitate were 2.6% and 0.3%, respectively. There were 17 kinds of hydrolyzed amino acids in vinegar sediment and vinegar ethanol precipitate. The vinegar sediment had 27.4% essential amino acids and 15.9% branched amino acids, while the vinegar ethanol precipitate had 19.5% essential amino acids and 7.7% branched amino acids. Therefore, the nutritional value of polypeptides in vinegar sediment is higher than that in vinegar ethanol precipitate. This study overall provides references for understanding the components of vinegar sediment.

Cite this article

GONG Tingting , LU Zhenming , GENG Yan , LONG Rong , WANG Haixiang , SHI Xiaohe , SHI Jinsong , XU Zhenghong . Analysis of polysaccharides and polypeptides in cereal vinegar sediment[J]. Food and Fermentation Industries, 2019 , 45(16) : 245 -249 . DOI: 10.13995/j.cnki.11-1802/ts.020518

References

[1] FUKUYAMA N, JUJO S, ITO I, et al. Kurozu moromimatsu inhibits tumor growth of Lovo cells in a mouse model in vivo[J]. Nutrition, 2007, 23(1): 81-86.
[2] SHIZUMA T, ISHIWATA K, NAGANO M, et al. Protective effects of fermented rice vinegar sediment (Kurozu moromimatsu) in a diethylnitrosamine-induced hepatocellular carcinoma animal model[J]. Journal of Clinical Biochemistry and Nutrition, 2011, 49(1): 31-35.
[3] NAGANO M, UENO T, FUJII A, et al. Anti-hyperglycemic effect of Kurozu moromi powder in type Ⅱ diabetic model KK-A mice[J]. Nippon Shokuhin Kagaku Kogaku Kaishi, 2010, 57(8): 346-354.
[4] FUJINO T, KANAYA S, ARIYOSHI K, et al. Effects of solid components in brewed vinegar on human serum cholesterol and red cell filtrability[J]. J Health Sci, 1990, 12: 139-141.
[5] FUJINO T, KANAYA S, KAJII R, et al. Effects of solid components in brewed vinegar on human serum lipids[J]. J Health Sci, 1993, 15: 91-93.
[6] HAYASHI T, HASEGAWA K, SASAKI Y, et al. Reduction of development of late allergic eosinophilic rhinitis by kurozu moromi powder in BALB/c mice[J]. Food Science and Technology Research, 2007, 13(4): 385-390.
[7] HAYASHI T, HASEGAWA K, SASAKI Y, et al. Amelioration of late asthmatic bronchial-bronchiolar lesions in BALB/c mice by kurozu moromi powder[J]. Food & Agricultural Immunology, 2006, 17(3-4): 139-148.
[8] SHIZUMA T, ISHIWATA K, NAGANO M, et al. Protective effects of Kurozu and Kurozu Moromimatsu on dextran sulfate sodium-induced experimental colitis[J]. Digestive Diseases and Sciences, 2011, 56(5): 1 387-1 392.
[9] OHKURA N, KIHARA-NEGISHI F, FUJII A, et al. Effects of fermented rice vinegar Kurozu and its sediment on inflammation-induced plasminogen activator inhibitor 1 (PAI-1) increase[J]. Food and Nutrition Sciences, 2018, 9: 235-246.
[10] NAGANO M, AOYAMA Y, HAYASHI K, et al. Effects of kurozu moromi powder on calcium absorption in ovariectomized osteoporosis model rats[J]. Jpn Pharmacol Ther, 2001,29:635-641.
[11] 刘兰涛,杨小兰.山西老陈醋醋泥冻干粉对高脂饮食小鼠的降血脂与抗氧化作用[J].食品科学,2015,36(9):141-145.
[12] 赵宁,李伟泽,张倩,等.白及多糖提取工艺优化[J].应用化工,2015,44(12):2 212-2 215.
[13] GB 5009.3—2010 食品中水分的测定[S].北京:中国标准出版社,2010.
[14] GB/T 15672—2009 食用菌中总糖含量的测定[S].北京:中国标准出版社,2009.
[15] 吴寒秋,赵丹,戚华文,等.高效阴离子交换色谱法检测山银花多糖的单糖组成[J].食品工业,2018,39(1):270-273.
[16] 郑岚,马耀宏,杨俊慧,等.黑蒜多糖与鲜蒜多糖结构特征及抗氧化活性的比较研究[J].食品科技,2018,43(4):194-200.
[17] GB 5009.5—2016 食品中蛋白质的测定[S].北京:中国标准出版社,2016.
[18] 欧阳道福,杨泽锐,郁晓艺,等.柱前衍生RP-HPLC法测定肽藻营养粉中游离氨基酸的含量[J].现代食品科技,2018,34(7):280-285;104.
[19] 刘有晴,黄丹,倪月,等.四川麸醋中氨基酸组分分析及营养评价[J].中国调味品,2016,41(5):72-75.
[20] 阿地拉·艾皮热,李金玉,付长霜,等.硫酸多糖生物活性研究进展[J].食品与生物技术学报,2019,38(1):13-19.
[21] 陈艳萍,张晓鸣.菌菇柄酶解物美拉德反应前后风味变化的研究[EB/OL].北京:中国科技论文在线,2012-08-03.
[22] 王先远,金宏,赵稳兴,等.补充支链氨基酸对运动大鼠心肌、骨骼肌代谢的影响[J].中国运动医学杂志,2002,21(1):98-99.
[23] 吕铮,孟庆华.肝硬化患者支链氨基酸的应用进展[J].临床肝胆病杂志,2015,31(3):475-477.
[24] 张倩.支链氨基酸治疗肝硬化的研究进展[D].重庆:重庆医科大学,2016.
Outlines

/