Sorghum distiller's grain is a cellulosic by-product from Chinese liquor fermentation. Dark fermentation is considered to be one of the most promising approaches for treating sorghum distiller's grain, along with biohydrogen production. To improve the hydrogen yield from sorghum distiller's grain, saccharification of sorghum distiller's grain and the hydrogen production rate were investigated. Results showed that the optimum approach for sorghum distiller's grain saccharification was to use enzymatic method, and the corresponding solid/liquid ratio and cellulase amount were 1:15 and 4 000 U/g, respectively. The obtained sugar yield reached 17.21%, which was 341.3% higher than that of the control group. Results of dark fermentation illustrated that a higher hydrogen yield (51.56 mL/g) was obtained with the addition of cellulase and amylase. Results of the scanning electron microscope showed that the surface structure of distiller's grains was significantly destroyed by the additional cellulase, indicating the conversion of cellulose to sugar was achieved.
YANG Li
,
SONG Yang
,
XU Xiaoyi
,
ZHANG Cunsheng
. Saccharification of sorghum distiller's grain and its performance for hydrogen production via dark fermentation[J]. Food and Fermentation Industries, 2020
, 46(1)
: 99
-103
.
DOI: 10.13995/j.cnki.11-1802/ts.021751
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