Effect of ultrasonic treatment on emulsification characteristics of water-soluble pig liver protein

  • KANG Mengyao ,
  • DING Jing ,
  • LU Xiaochuan ,
  • LI Yixuan ,
  • SHANG Yongbiao
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  • 1 (College of Food Science, Southwest University, Chongqing 400715, China)
    2 (Laboratory of Quality and Safety Risk Assessment for Agro-Products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing 400715, China)
    3 (Chongqing Engineering Research Center for Special Foods, Chongqing 400715, China)

Received date: 2019-08-14

  Online published: 2020-03-13

Abstract

This study is to explore the effect and mechanism of ultrasonic treatment on emulsification of water-soluble pig liver protein and provide a technical basis for the processing and utilization of pig liver protein. The water-soluble protein of pig liver was extracted and prepared into 2.5 mg/mL protein solution, which was respectively treated with 300, 360, 420, 480 and 540 W power ultrasonic treatment for 7 min and 420 W power ultrasonic treatment for 4, 7, 10, 13 and 16 min, and then emulsified. The results showed that ultrasonic emulsifying activity and emulsifying stability of pork liver water-soluble protein types and adsorption, surface tension, interfacial protein, droplet size and particle size distribution, emulsion milk analysis index and molecular reaction were significant. Moreover, the emulsifying performed the best when the ultrasonic power was 420 W, the processing time was 7 min, and emulsification and emulsion stability were 45.55 m2/g and 93.32%, respectively. In summary, ultrasonic treatment with appropriate strength and time can effectively improve the emulsification of water-soluble pig liver protein and improve the dispersion effect and molecular force of protein in aqueous solution.

Cite this article

KANG Mengyao , DING Jing , LU Xiaochuan , LI Yixuan , SHANG Yongbiao . Effect of ultrasonic treatment on emulsification characteristics of water-soluble pig liver protein[J]. Food and Fermentation Industries, 2020 , 46(3) : 144 -151 . DOI: 10.13995/j.cnki.11-1802/ts.021988

References

[1] 蔡育发. 以脏补脏 药膳五例[J]. 食品与生活, 2000(2): 24-25.
[2] 孙溥泉, 孙健慧. 猪肝的营养其它食物很难替代[J]. 养猪, 2009(1): 31-32.
[3] 刘兴江. 猪肝肠加工中的几个技术要求[J]. 肉类工业, 1992(4): 25-25.
[4] MARTIN D, RUIZ J, KIVIKARI R, et al. Partial replacement of pork fat by conjugated linoleic acid and/or olive oil in liver pâtés:Effect on physicochemical characteristics and oxidative stability[J].Meat Science, 2008,80(2):496-504.
[5] STEEN L, FRAEYE I, GOEMAERE O, et al. Effect of salt and liver/fat ratio on microstructure, emulsion stability, texture and sensory mouth feel of liver paste[J]. Food and Bioprocess Technology, 2014,7(10):2 855-2 864.
[6] 黄永洪, 花慧, 沈国强, 等. 猪肝过氧化氢酶提取条件的研究[J]. 生物加工过程, 2004,2(4):61-63.
[7] 黄静, 于素亚. 猪肝卵磷脂提取工艺研究[J]. 广东农业科学, 2010(1):105-106.
[8] JAYANI C, CHRISTINE O, SANDRA K,et al. Ultrasonics in food processing-Food quality assurance and food safety[J]. Trends in Food Science & Technology,2012,26(2):88-98.
[9] 李坚斌,李琳,陈玲,等.食品组分在超声场中的物性变化研究进展[J].食品科学,2007,28(10):558-561.
[10] HU Y Q,YU H X, DONG K C,et al. Analysis of the tenderisation of jumbo squid (Dosidicus gigas) meat by ultrasonic treatment using response surface methodology[J]. Food Chemistry,2014,160(10):219-225.
[11] BAHMAN N, MOHAMMAD A M, SAEEDEH S A, et al. Effect of ultrasound treatments on functional properties and structure of millet protein concentrate[J]. Ultrasonics-Sonochemistry,2018,41(2):382-388.
[12] NUCKLES R O, SMITH D M, MERKEL R A. Meat by-product protein composition and functional properties in model systems[J]. Journal of Food Science, 1990,55:640-643.
[13] STEEN L, GLORIEUX S, GOEMAERE O, et al. Functional properties of pork liver protein fractions[J]. Food and Bioprocess Technology, 2016, 9(6): 970-980.
[14] AGYARE K K, ADDO K, XIONG Y L. Emulsifying and foaming properties of transglutaminase-treated wheat gluten hydrolysate as influenced by pH, temperature and salt[J]. Food Hydrocolloids, 2009, 23(1): 72-81.
[15] PEARCE K N, KINSELLA J E. Emulsifying properties of proteins: evaluation of a turbidimetric technique[J]. Journal of Agricultural & Food Chemistry, 1978, 26(3): 716-723.
[16] SOLEIMANPOUR M, KOOCHEKI A, KADKHODAEE R. Influence of main emulsion components on the physical properties of corn oil in water emulsion: Effect of oil volume fraction, whey protein concentrate and Lepidium perfoliatum, seed gum[J]. Food Research International, 2013, 50(1): 457-466.
[17] 崔珊珊. 超高压处理甘薯蛋白酶解产物乳化特性的研究[D]. 乌鲁木齐:新疆农业大学, 2015.
[18] PALAZOLO G G, SOBRAL P A, WAGNER J R. Freeze-thaw stability of oil-in-water emulsions prepared with native and thermally-denatured soybean isolates[J]. Food Hydrocolloids, 2011, 25(3): 398-409.
[19] XIONG Y L, PARK D K, OOIZUMI T. Variation in the cross-linking pattern of porcine myofibrillar protein exposed to three oxidative environments[J]. Journal of Agricultural & Food Chemistry, 2009, 57(1): 153-159.
[20] GÓMEZ-GUILLÉN M C, BORDERIÍAS A J, MONTERO P. Chemical interactions of nonmuscle proteins in the network of sardine (Sardina pilchardus) muscle gels[J]. LWT-Food Science and Technology, 1997, 30(6): 602-608.
[21] 张涛. 鹰嘴豆分离蛋白的制备及其功能性质研究[D]. 无锡:江南大学, 2005.
[22] WONG B T, DAY L, AUGUSTIN M A. Deamidated wheat protein-dextran Maillard conjugates: Effect of size and location of polysaccharide conjugated on steric stabilization of emulsions at acidic pH[J]. Food Hydrocolloids, 2011, 25(6): 1 424-1 432.
[23] 徐文俊, 曹光群, 刘俊逸,等. 乳状液界面膜厚度与结构[J]. 无锡轻工业学院学报, 1987(2): 28-38;98-99.
[24] CHAN J, OMANA D A, BETTI M. Application of high pressure processing to improve the functional properties of pale, soft, and exudative (PSE)-like turkey meat[J]. Innovative Food Science & Emerging Technologies, 2011, 12(3): 216-225.
[25] REŽEK J, ANET L, VESNA M, et al. Physical properties of ultrasound treated soy proteins[J]. Journal of Food Engineering, 2009, 93(4): 386-393.
[26] LIU H, GAO L, REN Y, et al. Chemical interactions and protein conformation changes during silver carp (Hypophthalmichthys molitrix) surimi gel formation[J]. International Journal of Food Properties, 2014, 17(8):1 702-1 713.
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