In order to further improve the production of GABA synthesized by Enterococcus lactic acid faecalis, the effects of rotational speed (0 and 100 r/min) on the growth and GABA production of bacteria were investigated at a 250 mL shake flask level. By analyzing the fermentation curve and kinetic parameters at different rotational speeds, a two-stage rotational speed regulation strategy was proposed, and the fermentation conditions (stage time, liquid volume and rotational speed) that affected this strategy were optimized. It was found that the yield at 72 h and 0 r/min was the highest, and the biomass at 100 r/min in stable periods was the highest. The optimized fermentation conditions were as follows: the liquid volume was 100 mL, rotational speed was 150 r/min at the first stage of fermentation (0-12 h), then switched to 0 r/min at the second stage (12-72 h). On this basis, the production of GABA reached (6.713 ± 0.135) g/L, which was 12.2 % and 60.1 % higher than that before optimization at 0 and 100 r/min, respectively. Therefore, two-stage rotational speed control strategy can effectively improve the ability of E. lactic acid faecalis to synthesize GABA, which has an important reference value and guiding significance for industrial production of GABA.
ZHANG Min
,
XUE Zhenglian
,
YU Fei
,
LIU Yan
,
WANG Zhou
. Effects of two-stage rotational speed control on GABA production byEnterococcus lactic acid faecalis[J]. Food and Fermentation Industries, 2019
, 45(17)
: 20
-25
.
DOI: 10.13995/j.cnki.11-1802/ts.021048
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