This study aimed to screen microorganisms that can biodegrade waste oil in domestic sewage of Danjiangkou reservoir to reduce the pollution of water body of the reservoir caused by oil. Soybean oil was taken as the target pollutant, the strain enrichment and domestication were carried out, followed by neutral red plate screening and physiological and biochemical identification. A native microorganism was screened, which was identified as Proteus mirabilis and named P. mirabilis NY-201801. When 5% (v/v) oil and 10% inoculum size were cultivated for 36 h at pH 7.0, 35 ℃ and 150 r/min, P. mirabilis NY-201801 could degrade 92% oil. Meanwhile, at the highest water temperature in the reservoir area, the degradation rate of 5% (v/v) oil could reach 50%. Therefore, a new type of efficient oil-degrading bacteria with good environmental adaptability and application potential was obtained, which lays a solid foundation for biodegradation of waste oil in reservoir area.
SHI Hongling
,
CHEN Mengyan
,
LIU Fei
,
TANG Cunduo
,
LIU Yue
,
YAO Lunguang
,
KAN Yunchao
. Screening, identification and application of highly efficient waste oildegrading microorganisms[J]. Food and Fermentation Industries, 2019
, 45(17)
: 48
-53
.
DOI: 10.13995/j.cnki.11-1802/ts.020381
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