In order to evaluate the anti-oxidation and epidermal cell proliferation promotion of different concentrations of EBN water extract, EBN peptides and “95°fresh EBN” water extract, a H2O2-induced oxidative stress cell model was established based on the human embryonic lung fibroblasts (MRC-5). The content of reactive oxygen species (ROS) and malondialdehyde (MDA), and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. The results showed that the activities of SOD were significantly increased (P<0.05), while the levels of ROS were significantly reduced (P<0.05) after treated with EBN water extract, EBN peptides or “95° fresh EBN” water extract. In addition, the “95° fresh EBN” water extract could significantly increase the GSH-Px activity and reduce the MDA level compared with the other two groups. Meanwhile, all the three active EBN ingredients could stimulate the proliferation of human epidermal keratinocytes (Hacat) at the concertration of 100-200 μg/mL. Compared with traditional EBN water extract, “95° fresh EBN” water extract could significantly increase the proliferation of Hacat to 112.57% of the control group at the concentration of 200 μg/mL, which had the same effect with the EBN peptides.
DONG Jianhui
,
TIAN Qiaoji
,
DUAN Sufang
,
LIU Yifeng
,
LI Haizhi
,
XIA Kai
. Comparative study on anti-oxidation and promotion activity of epidermal cellgrowth of edible bird′s nest water extract[J]. Food and Fermentation Industries, 2019
, 45(17)
: 73
-78
.
DOI: 10.13995/j.cnki.11-1802/ts.021969
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