Proteases are a kind of important industrial enzymes. Rapid determination of their activity is very important for exploring, producing and applying new enzymes. Consequently, a novel method for rapid determination of protease activity was developed based on the principle of fluorescence energy resonance transfer using a fluoroboron fluorescein labeled soybean protein isolate as the substrate. The method was used to detect five commonly used industrial proteases and showed a good linearity in the range of 0.025-0.4 U/mL. Besides, the ranges of detection limit and quantitative limit were 1.1×10-4-1.4×10-4 U/mL and 3.7×10-4-4.5×10-4 U/mL, respectively. The sensitivity of this method was 20 000 times higher than that of Folin method. Therefore, the simplicity, sensitivity and stability of the method indicate its potential applications in rapid assay, trace detection and exploration of new proteases.
SONG Peng
,
CHENG Lei
,
TIAN Kangming
,
WANG Zhengxiang
. A novel method for rapid determination of industrial protease and itspreliminary application[J]. Food and Fermentation Industries, 2019
, 45(17)
: 216
-220
.
DOI: 10.13995/j.cnki.11-1802/ts.021134
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