Food and Fermentation Industries >

2019 , Vol. 45 >Issue 18: 195 - 201

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.020979

Isolation and identification of protease-producing bacterium in intestinal tract of sea cucumbers and its applications in treating sea cucumber residues

  • LI Zhen ,
  • XIAO Yang ,
  • ZHEN Tianyuan ,
  • PIAO Meizi
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  • College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China

Online published: 2019-11-06

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Abstract

A protease-producing bacterium from sea cucumber intestinal tract was isolated and identified. Through morphological, physiological, biochemical characterization and 16S rDNA sequence homology analysis, the isolated strain was identified as Thalassobacillus sp. QDHF-1. Sea cucumber intestine waste was fermented by strain QDHF-1, and the fermentation conditions were optimized and the antioxidant activities of produced peptides were determined. The optimal fermentation condition to produce peptides was 6% inoculum, 20% raw materials and 36 h fermentation. The polypeptides could scavenge ·OH, DPPH·, and O-2·, and the highest scavenging rate of 93.94% was obtained on ·OH using 50 g/L polypeptides. The results indicate the potential applications of strain QDHF-1 in sea cucumber offal fermentation to produce bioactive peptides.

Key words： sea cucumber intestine waste; protease-producing bacterium; fermentation; peptide; antioxidant activity

Cite this article

LI Zhen , XIAO Yang , ZHEN Tianyuan , PIAO Meizi . Isolation and identification of protease-producing bacterium in intestinal tract of sea cucumbers and its applications in treating sea cucumber residues[J]. Food and Fermentation Industries, 2019 , 45(18) : 195 -201 . DOI: 10.13995/j.cnki.11-1802/ts.020979

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