Research Report

Optimized condition for biosynthesizing α-glucosidase inhibitor in Paenibacillus bovis BD3526

  • HAN Jin ,
  • WU Zhengjun ,
  • LIU Zhenmin ,
  • ZHAO Yong
Expand
  • 1(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)
    2(Shanghai Engineering Research Center of Dairy Biotechnology, State Key Laboratory of Dairy Biotechnology,Dairy Research Institute, Bright Dairy and Food Co. Ltd., Shanghai 200436, China)

Revised date: 2017-07-10

  Online published: 2019-11-15

Abstract

This study was constructed to increase the yield of α-glucosidase inhibitor in fermented milk by Paenibacillus bovis BD3526. The fermentation parameters were optimized by single-factor experiments and response surface method. The results showed that the optimal condition for BD3526 to synthesize α-glucosidase inhibitor was as follows: cultivated at 29 ℃ for 43 h in a 50 mL medium with 5 g/100 g H2O skimmed milk in a 250 mL flask. Under the optimized condition, the IC50 of BD3526 fermented milk to inhibit α-glucosidase was 15.1 mg/mL, which agreed with the predicted value (14.6 mg/mL) and was 33% lower than that before optimization. In conclusion, this study investigated the optimized process of preparing α-glucosidase inhibitor in fermented skimmed milk by microorganisms for the first time, which provides a guarantee for subsequent separation of α-glucosidase inhibitor and also lays a foundation for other similar studies.

Cite this article

HAN Jin , WU Zhengjun , LIU Zhenmin , ZHAO Yong . Optimized condition for biosynthesizing α-glucosidase inhibitor in Paenibacillus bovis BD3526[J]. Food and Fermentation Industries, 2019 , 45(19) : 45 -51 . DOI: 10.13995/j.cnki.11-1802/ts.021332

References

[1] FOWLER, 徐赫男. 糖尿病分类:并非所有高血糖都一样[J]. 糖尿病天地(临床), 2008, 2(3): 103-105.
[2] 于彩丽. Ⅱ型糖尿病的药物治疗进展[J]. 中国医药指南, 2012, 10(29): 66-67.
[3] ADEMILUYI A O, OBOH G.Soybean phenolic-rich extracts inhibit key-enzymes linked to type 2 diabetes (alpha-amylase and alpha-glucosidase) and hypertension (angiotensin I converting enzyme) in vitro[J]. Experimental and Toxicologic Pathology, 2013, 65(3): 305-309.
[4] 季芳, 肖国春,董莉, 等. 药用植物来源的α-葡萄糖苷酶抑制剂研究进展[J]. 中国中药杂志, 2010, 35(12): 1 633-1 640.
[5] 卜同, 陈雷,林秀坤. 天然来源的α-葡萄糖苷酶抑制剂的研究进展[J]. 海洋科学, 2010, 34(2): 92-95.
[6] 韩瑨, 季红,周方方, 等. 产α-葡萄糖苷酶抑制剂乳酸菌的筛选及发酵条件优化[J]. 天然产物研究与开发, 2013, 25(3): 302-306.
[7] 张欢欢, 韩瑨,游春苹, 等. 植物乳杆菌来源的α-葡萄糖苷酶抑制剂的分离纯化[J]. 应用化工, 2015, 44(8): 1 417-1 420.
[8] CHAPDELAINE P, TREMBLAY R R, DUBE J.P-Nitrophenol-alpha-D-glucopyranoside as substrate for measurement of maltase activity in human semen[J]. Clinical Chemistry, 1978, 24(24): 208-211.
[9] 范文娅, 吴正钧,郭本恒. 响应面法优化干酪乳杆菌LC2W代谢产α-葡萄糖苷酶抑制剂的发酵条件[J]. 江苏农业科学, 2012, 40(10): 228-231.
[10] 苍桂璐, 张付云,杨阳, 等. 多粘类芽孢杆菌的研究进展[J]. 安徽农业科学, 2013, 399(2): 487-489.
[11] GRADY E N, MACDONALD J, LIU L, et al.Current knowledge and perspectives of Paenibacillus: a review[J]. Microbial Cell Factories, 2016, 15(1): 203-220.
[12] GAO C, HAN J, LIU Z, et al.Paenibacillus bovis sp. nov., isolated from raw yak (Bos grunniens) milk[J]. International Journal of Systematic & Evolutionary Microbiology, 2016, 66(3): 1 413-1 418.
[13] 杭锋, 洪青,陶源, 等. Paenibacillus spp.BD3526发酵小麦麸皮生产凝乳酶[J]. 食品与发酵工业, 2016, 42(2): 35-40.
[14] HANG F, LIU P, WANG Q, et al.High milk-clotting activity expressed by the newly isolated Paenibacillus spp. Strain BD3526[J]. Molecules, 2016, 21(1): 73-86.
[15] HANG F, WANG Q, HONG Q, et al.Purification and characterization of a novel milk-clotting metalloproteinase from Paenibacillus spp. BD3526[J]. International Journal of Biological Macromolecules, 2016, 85: 547-554.
[16] HANG F, WANG Q, HONG Q, et al.Structural insight into a novel neutral metalloproteinase from Paenibacillus spp. BD3526: Implications for mechanisms of rapid inactivation and calcium-dependent stability[J]. International Journal of Biological Macromolecules, 2017, 95: 1 082-1 090.
[17] 杭锋, 洪青,王钦博. Paenibacillus spp. BD3526金属蛋白酶对乳蛋白水解位点分析[J]. 乳业科学与技术, 2016, 39(5): 1-6.
[18] 崔燕丽, 韩瑨,吴正钧. Paenibacillus bovis BD3526产胞外多糖的培养条件优化[J]. 食品与发酵工业, 2016, 42(5): 108-113.
[19] 徐晓芬. 类芽孢杆菌BD3526在无氮固体培养基上产胞外多糖的研究[J]. 食品工业科技, 2017, 38(16): 95-100.
[20] XU X, GAO C, LIU Z, et al.Characterization of the levan produced by Paenibacillus bovis sp. nov BD3526 and its immunological activity[J]. Carbohydr Polym, 2016, 144: 178-186.
[21] 刘玉娟, 韩瑨,吴江, 等. 类芽孢杆菌BD3526抑菌活性物质的初步分离及性质测定[J]. 天然产物研究与开发, 2015, 27(7): 1 176-1 180.
[22] 花榜清, 张秋香,刘振民,等. 牛类芽孢杆菌BD3526产抗菌物质培养条件的优化[J]. 食品与机械, 2017, 33(3): 31-36.
[23] 洪青, 杭锋,吴正钧. 类芽孢杆菌BD3526发酵上清对植物乳杆菌ST-Ⅲ的促生长研究[J]. 乳业科学与技术, 2016, 39(6): 1-5.
Outlines

/