This study was constructed to increase the yield of α-glucosidase inhibitor in fermented milk by Paenibacillus bovis BD3526. The fermentation parameters were optimized by single-factor experiments and response surface method. The results showed that the optimal condition for BD3526 to synthesize α-glucosidase inhibitor was as follows: cultivated at 29 ℃ for 43 h in a 50 mL medium with 5 g/100 g H2O skimmed milk in a 250 mL flask. Under the optimized condition, the IC50 of BD3526 fermented milk to inhibit α-glucosidase was 15.1 mg/mL, which agreed with the predicted value (14.6 mg/mL) and was 33% lower than that before optimization. In conclusion, this study investigated the optimized process of preparing α-glucosidase inhibitor in fermented skimmed milk by microorganisms for the first time, which provides a guarantee for subsequent separation of α-glucosidase inhibitor and also lays a foundation for other similar studies.
HAN Jin
,
WU Zhengjun
,
LIU Zhenmin
,
ZHAO Yong
. Optimized condition for biosynthesizing α-glucosidase inhibitor in Paenibacillus bovis BD3526[J]. Food and Fermentation Industries, 2019
, 45(19)
: 45
-51
.
DOI: 10.13995/j.cnki.11-1802/ts.021332
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