A new method for screening 154 pesticide residues in celery and tomato was established by UPLC-Q-TOF/MS. The QuEChERS method was used for sample preparation, and the sample was extracted with acetonitrile solution containing 1% (V/V) acetic acid and salted out with anhydrous magnesium sulfate and sodium chloride. The extract was purified by adsorption material and then determined directly. The target drug was separated by C18 column and gradient elution was performed with acetonitrile and 0.1% (V/V) formic acid aqueous solution as mobile phase. Full information tandem mass spectrometry (MSE) with positive ion was used to detect the target drug. The results showed that in the range of 1-100 μg/kg, the 154 pesticides in two vegetables showed good linear correlations (R2≥0.995), and the screening detection limits (SDLs) and limits of quantification (LOQs) of 154 pesticides in the two vegetables were both in the range of 1-100 μg/kg. The average recoveries for 154 pesticides at three spiked levels ranged from 52% to 127%, with relative standard deviations(RSDs) of 0.3%~39.0%. The method is rapid, simple and sensitive, which can be used for rapid screening of 154 pesticide residues in celery and tomato.
LI Jianxun
,
FAN Bei
,
ZHOU Jie
,
ZHEN Yunpeng
,
SHAN Jihao
. Rapid screening of 154 pesticide residues in vegetables by QuEChERS-ultra high performance liquid chromatography-time mass spectrometry[J]. Food and Fermentation Industries, 2019
, 45(19)
: 239
-250
.
DOI: 10.13995/j.cnki.11-1802/ts.021094
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