Food and Fermentation Industries >

2019 , Vol. 45 >Issue 19: 292 - 298

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.021238

Overview and special review

Mechanisms of specific substrate hydrolysis of proteases: a review

  • XIONG Ke ,
  • DENG Lei ,
  • LIU Jiayun ,
  • PEI Penggang
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  • 1(Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University, Beijing 100048, China)
    2(Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University, Beijing 100048, China)
    3(Beijing Key Laboratory of Flavor Chemistry,Beijing Technology and Business University, Beijing 100048, China)
    4(Beijing Laboratory for Food Quality and Safety, Beijing Technology & Business University, Beijing 100048, China)

Revised date: 2019-07-01

  Online published: 2019-11-15

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Abstract

The specificity of enzyme hydrolysis site of proteases has a great influence on the composition and ratio, as well as the value of the products. Enzymatic hydrolysis of proteins has the disadvantages of limited efficiency, low yield of functional specific components and high purification cost, which limit the large-scale industrial application. In order to solve the problems, it is necessary to elucidate the molecular mechanisms of how proteases can specifically hydrolyze substrates. This review was therefore conducted to discuss the mechanisms from several aspects, including the properties of substrate groups, the characteristics and spatial position of amino acids at binding sites, as well as the surface charge of enzyme molecules etc. It was found that the basic catalytic mechanism of proteases could not explain the differences in substrate specificity. At present, several researches on the mechanisms of protease specificity provided guiding significance, but a comprehensive and unified mechanism has not yet been formed, which needs further research and improvement.

Key words： protease; enzymatic hydrolysis mechanism; specificity; group properties; binding site; surface charge

Cite this article

XIONG Ke , DENG Lei , LIU Jiayun , PEI Penggang . Mechanisms of specific substrate hydrolysis of proteases: a review[J]. Food and Fermentation Industries, 2019 , 45(19) : 292 -298 . DOI: 10.13995/j.cnki.11-1802/ts.021238

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