The trinitrobenzenesulfonic acid (TNBS) method is a kind of method for determining the content of free amino groups in proteins. This paper compared two termination reaction conditions and analytical methods, and explored the applicability of the method for different proteins (bovine serum albumin, trypsin, lysozyme). The results showed that (a) the response value under alkaline conditions was significantly higher than that of acidity (P<0.01); (b) the results of UV-visible spectrophotometer and microplate reader showed no significant difference in a certain range(P>0.05); (c) the accuracy of the ultraviolet-visible spectrophotometer was higher and the response to the changes in concentration was more sensitive than control group. Now only did protein species affect the sampling concentration, but also the sulfhydryl content in the proteins interfered with the accuracy of the method.
CHU Qianqian
,
HAN Qiuyu
,
CHEN Biwen
,
BAO Bin
. Determination of free amino group content of protein using trinitrobenzenesulfonic acid (TNBS) method[J]. Food and Fermentation Industries, 2020
, 46(6)
: 275
-279
.
DOI: 10.13995/j.cnki.11-1802/ts.021275
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