In order to reduce the running cost, immobilization of β-fructofuranosidase Fru6 was investigated for fructo-oligosaccharide preparation. Fru6 was immobilized by multi-functional resin MI-BN4 and the immobilization condition was optimized. The enzymatic properties, the ability to catalyze fructo-oligosaccharide and the capacity for reusing immobilized Fru6 were explored. When adsorbed for 6 h at 25 °C with 40 U enzyme, an immobilized enzyme with 205.7 U/g activity was obtained, which had 95.2% recovery rate of enzyme activity and absorbed 0.6 mg/g dry resin protein. Compared with free enzyme, the fructo-oligosaccharides conversion rate with immobilized Fru6 increased from 62% to 72% and the yield of fructo-oligosaccharides slightly reduced. Besides, the immobilized Fru6 could be reused for 12 times. Overall, the immobilized Fru6 has high enzyme activity recovery rate and good catalytic ability, which lays a foundation for fructo-oligosaccharides production.
CHENG Tiantong
,
HE Bingfang
. Immobilization of β-fructofuranosidase Fru6 by macroporous resin MI-BN4 and its application in catalytic synthesis of fructo-oligosaccharides[J]. Food and Fermentation Industries, 2019
, 45(20)
: 16
-21
.
DOI: 10.13995/j.cnki.11-1802/ts.021254
[1] 熊伟,张健. 低聚果糖(FOS)-超强双歧因子[J]. 宁夏师范学院学报, 2005,26(6):292-294.
[2] LORENZONI A S G, AYDOS L F, KLEIN M P, et al. Fructooligosaccharides synthesis by highly stable immobilized β-fructofuranosidase from Aspergillus aculeatus[J]. Carbohydrate Polymers, 2014,103:193-197.
[3] NAWAZ A, BAKHSH J A, IRSAD S, et al. The functionality of prebiotics as immunostimulant: Evidences from trials on terrestrial and aquatic animals[J]. Fish Shellfish Immunol, 2018,76:272-278.
[4] SHEU D C, CHANG J Y, CHEN Y J, et al. Production of high-purity neofructooligosaccharides by culture of Xanthophyllomyces dendrorhous[J]. Bioresource Technology, 2013,132(3):432-435.
[5] BRUNO S N F,VAITSMAN D S,KUNIGAMI C N,et al.Influence of the distillation processes from Rio de Janeiro in the ethyl carbamate formation in Brazilian sugar cane spirits[J].Food Chemistry,2007,104(4):1 345-1 352.
[6] KILIAN S, KRITZINGER S, RYCROFT C, et al. The effects of the novel bifidogenic trisaccharide, neokestose, on the human colonic microbiota[J]. World Journal of Microbiology & Biotechnology, 2002,18(7):637-644.
[7] 苏汇,陈琼珍,何冰芳. 耐有机溶剂果糖苷酶的胞外表达、纯化和结晶[J]. 微生物学通报, 2015,42(11):2 126-2 132.
[8] 何冰芳.β-呋喃果糖苷酶突变体及其应用:中国, CN 201810405006.5 [P]. 2018-09-28.
[9] MATEO C, BOLIVAR J M, GODOY C A, et al. Improvement of enzyme properties with a two-step immobilizaton process on novel heterofunctional supports[J]. Biomacromolecules, 2010,11(11):3 112-3 117.
[10] LARRE S, CAMPARO P, COMPERAT E, et al. Diagnostic, staging, and grading of urothelial carcinomas from urine: Performance of BCA-1, a mini-array comparative genomic hybridisation-based test[J]. Eur Urol, 2011,59(2):250-257.
[11] GUERRERO C, VERA C, SERNA N, et al. Immobilization of Aspergillus oryzae β-galactosidase in an agarose matrix functionalized by four different methods and application to the synthesis of lactulose[J]. Bioresource Technology, 2017,232:53-63.
[12] RODRIGUES R C, ORTIZ C, BERENGUER-MERCIA A, et al. Modifying enzyme activity and selectivity by immobilization[J]. Chem Soc Rev, 2013,42(15):6 290-6 307.
