In order to reduce the cost of industrial production of γ-decalactone (GDL), the feasibility of kitchen waste oil as carbon sourcewas studied. Equivalent biomass of Yarrowia lipolytica in complete medium was gained after 12 h cultivation under the optimized medium consisted of 12.5 g/L glucose, 5 g/L yeast extract and 6 g/L kitchen waste oil. Engineered Y. lipolytica overexpressing acetyl-CoA oxidase gene pox2 produced 1.5g/L of GDL from ricinoleic acid was, which was 1.2 times higher than that of parent strain. Y. lipolytica CICC31223 with high lipase-producing ability was co-cultured with the engineered strain for efficient degradation of waste oil and transforming castor oil into GDL. The optimal production conditions were determined as follows: inoculation ratio of Y. lipolytica CICC31223 to Y. lipolytica was 1∶10 (V/V), Y. lipolytica was inoculated after culturing Y. lipolytic CICC31223 for 12 h at 28 ℃ at 200 r/min. It was found that the level of GDL reached 0.15 g/L at above optimal conditions and it was significantly higher than single culture production (0.08 g/L). The results suggested that the kitchen waste oil is an economical carbon source and producing GDL by mixed fermentation of yeasts has great industrial application prospects.
WANG Rongxia
,
ZHU Tingheng
,
WANG Kun
. Biotransformation of γ-decalactone from kitchen waste oil by yeasts[J]. Food and Fermentation Industries, 2019
, 45(20)
: 106
-111
.
DOI: 10.13995/j.cnki.11-1802/ts.020676
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