Natto polysaccharide is one of the main functional substances in natto. The content of physicochemical components of natto polysaccharides were determined. Meanwhile, the molecular weight and monosaccharide composition were analyzed by high performance liquid chromatography and gas chromatography-mass spectrometry. The functional groups and spatial structure were determined by Fourier transform infrared spectroscopy and the Congo red test. The results showed that percentage of each component in natto polysaccharides was 66.01% of total sugar, 1.12% of protein, 2.79% of uronic acid and 1.57% of sulfate. After purification, natto polysaccharides was found to be a single component, with molecular weight of 11.53 kDa. The molar ratio of fucose∶xylitol∶mannose∶glucose∶galactose was 1.04∶1.53∶1.57∶1.19∶4.68. In addition, infrared spectrum analysis showed that natto polysaccharides had characteristic absorption peak of polysaccharides, and the Congo red experiment revealed that natto polysaccharides had triple helix structure. The in-depth analysis of the physicochemical properties and structure of natto polysaccharides can provide a theoretical basis for the selection of functional plant polysaccharides.
YNAG Wenli
,
YNAG Bo
,
YANG Guang
. Physicochemical properties and structural analysis of natto polysaccharides[J]. Food and Fermentation Industries, 2019
, 45(20)
: 132
-137
.
DOI: 10.13995/j.cnki.11-1802/ts.020681
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