A rapid method for the determination of vitamin D in infant formula and infant rice has been developed using online SPE column and two -dimensional liquid chromatography. The injected sample solution was pre-treated by hot saponification using KOH solution and ethanol. The sample solution was purified by an online solid phase extraction column through a six-way valve, then the purification liquid entered one-dimensional chromatography. The one-dimensional chromatography used the C8 chromatography column for preliminary separation. The heart-cutting time widow of vitamin D was confirmed according to the retention time of vitamin D in the first dimensional separation. During this period, the effluents were transferred to another six-way valve for further separation by two-dimensional chromatography to achieve rapid determination of vitamin D in infant formula and infant rice. The standard addition recovery of vitamin D by this method was 91.3% to 97.2%, and the relative standard deviation was less than 5%. There was no statistically significant difference between this method and standard method through t-test. The method was simple, fast and accurate, which was suitable for the determination of large quantities of samples.
YE Jiaming
,
WANG Jing
,
YE Leihai
,
HE Bin
,
YANG Haiqing
,
WANG Qingqi
,
CHEN Qingjun
. Determination of vitamin D in infant formula and rice flour by online SPE column and two-dimensional liquid chromatography[J]. Food and Fermentation Industries, 2020
, 46(7)
: 239
-243
.
DOI: 10.13995/j.cnki.11-1802/ts.022221
[1] 赵玉富,咸伟玥.二维液相色谱法同时测定饲料中维生素A、维生素D3、维生素E[J].饲料博览,2019(4):51-54.
[2] 赵玉富,刘晓玲,咸伟玥,等.二维液相色谱同时测定婴幼儿配方乳粉中多种脂溶性维生素[J].中国乳品工业,2019,47(6):37-40.
[3] 林玉宙,吴宏,郭荣烁,等.二维液相色谱/双检测器法同时测定婴幼儿配方奶粉、米粉中维生素A、D3和E的含量[J].食品工业科技,2016,37(20):68-71;77.
[4] 储小军,陶保华,赖世云,等.固相萃取-二维液相色谱法测定婴幼儿配方食品中的维生素B12[J].浙江大学学报(农业与生命科学版),2013,39(2):185-190.
[5] 陈静. 二维高效液相色谱法测定营养饮料中的水溶性维生素[A]. 中国化学会色谱专业委员会、北京色谱学会.第十届全国生物医药色谱及相关技术学术交流会论文集[C].中国化学会色谱专业委员会、北京色谱学会:中国化学会,2014:2.
[6] 陈静. 二维色谱中心切割技术在水溶性维生素分析中的应用研究[D].上海:复旦大学,2014.
[7] 黄静,张毅.高效液相色谱法测定二维钙赖氨酸片中维生素B1含量[J].中国药业,2006(11):32-33.
[8] CHEN Yingzhuang, CHEN Bo,et al.Pulsed elution modulation for on-line comprehensive two-dimensional liquid chromatography coupling reversed phase liquid chromatography and hydrophilic interaction chromatography[J].Journal of Chromatography A,2019,1 583:98-107.
[9] GUO Pengqi, LUO Zhimin, FU Qiang,et al.Development of molecular imprinted column-on line-two dimensional liquid chromatography for selective determination of clenbuterol residues in biological samples[J]. Food Chemistry,2017,217:628-636.
[10] JI Shuai, WANG Shuang, YE Min,et al.The application of on-line two-dimensional liquid chromatography (2DLC) in the chemical analysis of herbal medicines[J].Journal of Pharmaceutical and Biomedical Analysis,2018,160:301-313.
[11] ANDREA MARTÍN-ORTIZ, ANA ISABEL RUIZ-MATUTE, MARÍA LUZ SANZ, et al. Separation of di- and trisaccharide mixtures by comprehensive two-dimensional liquid chromatography. Application to prebiotic oligosaccharides[J].Analytica Chimica Acta ,2019,1 060:125-132.
[12] MA Jing, HOU Xiaofang, ZHANG Bing, et al.The analysis of carbohydrates in milk powder by a new “heart-cutting” two-dimensional liquid chromatography method[J].Journal of Pharmaceutical and Biomedical Analysis,2014,91:24-31.
[13] 张艳海,其布勒哈斯,等.在线二维液相色谱法同时测定婴幼儿和成人配方营养品中的维生素A、D3和E[J].色谱,2015,33(3):291-297.
[14] 张艳海,朱晓艳,曹国洲,等.在线二维柱切换-高效液相色谱法同时测定婴幼儿强化奶粉中维生素A、D3和E的含量[J].分析化学,2013,41(5):771-775.
[15] 张艳海,张大伟,曹莹,等.在线二维反相色谱法快速测定维生素AD制剂中维生素A、D的含量[J].分析测试学报,2016,35(1):28-34.
[16] 张艳海. 在线二维液相色谱法快速同时测定婴幼儿配方奶粉中维生素A,D和4种VE异构体的含量[A]. 中国化学会.第21届全国色谱学术报告会及仪器展览会会议论文集[C].中国化学会:中国化学会,2017:412-413.
[17] 戚绿叶.柱切换HPLC法测定保健食品中5种维生素A和D成分的研究[D].杭州:浙江工业大学,2015.
[18] 戚绿叶,颜琳琦,张丽媛,等.二维液相色谱法测定保健食品中VA、V(D3)、VE的含量[J].中国卫生检验杂志,2015,25(3):340-343;347.
[19] 陈建彪.在线净化二维液相色谱快速检测食品中维生素A、D、E[J].食品安全质量检测学报,2019,10(6):1 726-1 733.
[20] 岑建斌,区硕俊,周朗君,等.二维液相色谱法同时检测婴幼儿乳粉中维生素A、E与D3[J].中国乳品工业,2017,45(2):43-46.
[21] 中华人民共和国国家标准 GB 5009.82—2016食品安全国家标准-食品中ADE的测定[S].
