The aim of this study was to better exert the effects of Bifidobacterium on human health. The mixed solution of Bifidobacterium and oligofructose was used as the core material, sodium alginate and whey protein were used as wall materials, and Bifidobacterium microcapsules were prepared by endogenous emulsification. The embedding rate of wet capsules was used as an evaluation index, and the optimal conditions for preparation of Bifidobacterium microcapsules were determined by single factor and response surface tests. The experiment results showed that when the concentration of sodium alginate was 2 %, the mass ratio of whey protein to sodium alginate was 2:1, the mass ratio of calcium carbonate to sodium alginate was 1:2, the volume ratio of water phase to oil phase was 2:5, and the glacial acetic acid was 400 μL, the moment embedding rate of the Bifidobacterium wet microcapsules can reach a maximum of 81.15%. The wet capsules obtained under the optimal process conditions were freeze-dried to make dry capsules, and tested for tolerance to gastric acid and bile salt and intestinal release and digestive system, using the free bacteria as the control group. After the experiment of simulated digestive juice, it was found that the number of live bacteria per milliliter was higher than that of free bacteria. The result showed that, the physical and chemical properties of Bifidobacterium microcapsules prepared by optimized strip were better than that of free bacteria.
[1] SARAO L K,ARORAM.Probiotics,prebiotics,and microencapsula-tion:Areview[J].Crit rev foogsci Nutr,2017,57(2):344-371.
[2] 陈婷,王玉华,蔡丹,等.益生菌微胶囊技术研究进展[J].中国乳品工业,2016,44 (1):32-37.
[3] 曾睿,邹悦瑜,王歌,等.4种食品中5-羟甲基糠醛在模拟胃肠消化过程中的变化[J]. 食品与发酵工业,2019,45(1):83-87.
[4] 胡锐,周广萍,宋维春.中药益生元微胶囊饲料添加剂对斜带石斑鱼生长和免疫性能的影响[J].饲料研究,2017,39(1):5-12;37.
[5] 吕锡斌,何腊平,张汝娇,等.双歧杆菌生理功能研究进展[J].食品工业科技,2013, 34(16):353-358.
[6] 梁永海,李凤林,庄威,等.红小豆双歧杆菌发酵保健饮料生产工艺的研究[J].冷饮与速冻食品工业,2005,11(4):18-20.
[7] 李来酉.双歧杆菌等三联益生菌培养条件及微囊化的研究[D].哈尔滨:东北林业大学,2009.
[8] BARBA-VIDALE, CASTILLEJOSL, ROLLVFB, et al. Infantis CECT7210 and Bifidobacterium animal issubsp[J].Front Microbiol,2017,8:1 568-1 570.
[9] 宋娇娇,王俊国,田文静,等.微胶囊制备工艺对益生菌活性的影响[J].中国乳品工业,2016,44(1):25-30.
[10] CAILLARD R LAPOINTEN.In vitro gastric survival of commercially available probiotic strains and oral dosage forms[J].Int J Pharm,2017,519(1-2):125-127.
[11] 李康宁,邢会霞,焦志军,等.双歧杆菌NQ1501双层微胶囊制备及其特性研究[J].中国微生态学杂志,2016,28(11):1 246-1 248;1 252.
[12] 杨剀舟,刘云,杨立芳,等.长双歧杆菌BBMN68微胶囊的制备及其应用性评价[J].食品科学,2015,36(9):71-77.
[13] ETCHEPARE M D A,RADDATZ G C, FLORES M D M, et al. Effect of resistant starch and chitosan on survival of Lactobacillus acidophilus, microencapsulated with sodium alginate[J].LWTFood Science and Technology, 2016, 65: 511-517.
[14] 孙玉章,崔栩,马丰英,等.益生菌微胶囊化技术研究进展[J].动物医学进展, 2018,39(7):86-89.
[15] 赵萌,蔡沙,屈方宁,等.内源乳化法制备海藻酸盐微胶囊的研究进展[J].食品工业科技,2013,34(22):392-396.
[16] 阳晖,蒋爱民.内源乳化法制备肠溶性嗜酸乳杆菌微胶囊的研究[J].食品研究与开发,2008,29(6):57-60.
[17] SONG H, YU W, GAO M, et al. Microencapsulated probiotics using emulsification technique coupled with internal or external gelation process[J].Carbohydrate Polymers,2013,96:181-189.
[18] 刘若男.内源乳化法制备溶菌酶肠溶性微胶囊及所用油相回收的研究[D].无锡:江南大学,2011.
[19] 宋娇娇,包秋华,王亚利,等.瑞士乳杆菌MG9-2微胶囊的制备工艺优化及其性能分析[J].食品科学,2017,38(14):49-57.
[20] ZHANG F, ZHAO M ,WANG W, et al. Encapsulation of Bifidbacteriumbifidum in improved alginate microcapsules toprolonging viability[J].Materials Research, 2011:1 481-1 485.
[21] 邹强,梁华忠,龚春雪,等.海藻酸钠和乳清蛋白作为益生菌包埋壁材的比较[J].食品科学,2014,35(15):207-211.
[22] HEIDEBACH T, FORST P, KULOZIK U. Influence of casein-based microencapsulation on freeze-drying and storage of probiotic cells[J]. Journal of Food Engineering, 2010, 98(3): 309-316.
[23] GOMEZ-MASCARAQUE L G, MORFIN R C, PÉREZ-MASIÁ R, et al. Optimization of electrospraying conditions for the microencapsulation of probiotics and evaluation of their resistance during storage and in vitro digestion[J]. LWT-Food Science and Technology, 2016, 69: 438-446.
[24] 邓玉娣,霍蔼倩,黎锶华,等.双歧杆菌复合微胶囊的工艺优化、表征及功能特性分析[J].食品工业科技,2019,40(6):147-156.
[25] 张国芳,王婷婷,刘丽波,等.内源乳化法制备干酪乳杆菌微胶囊[J].中国乳品工业,2017,45(3):15-20.
[26] 张生生,闫静芳,陆兆新,等.锐孔凝固浴法制备表面活性肽微胶囊食品工业科技[J].食品工业科技,2015,35(12):221-230.
[27] 陈丽,张喻,谭亦成,等.双层包埋制备大米抗氧化肽微胶囊工艺研究[J].中国食品学报,2014,14(1):134-139.
[28] 王伟洁,秦翠丽,王大红,等.响应面法优化保加利亚乳杆菌微胶囊制备工艺[J].中国酿造,2018,37(9):105-109.
[29] 周艳林,吴影,马雨浩,等.魔芋葡甘聚糖-海藻酸钠复合益生菌微胶囊的构建及性能评价[J].食品与发酵工业,2019,45(20):52-60.
