In order to improve the tolerance of ethanologenic Escherichia coli to substrate and ethanol and the performance of ethanol fermentation, the trehalose metabolic pathway of E. coli B0013-1031H was engineered and the mutants JC31 and JC41 were constructed. In strain JC31, the trehalose catabolism pathways were deleted, the trehalose synthesis pathway was further enhanced and resulted in strain JC41. The ability of trehalose synthesis and accumulation of JC31 and JC41 was higher than that of the original strain, in which, JC41 intracellularly accumulated trehalose up to 12-fold that of the original strain. The tolerance of JC31 and JC41 to glucose and ethanol stress was significantly improved. The ethanologenic recombinant E. coli JC31-PA obtained by engineering ethanol synthesis pathway fermented 120 g/L of glucose and yielded 50.6 g/L of ethanol, which was 5.42% higher than that of the control strain. The conversion rate of ethanol from glucose was 48.72 g/100 g glucose, which was 12.67% higher than that of the control.
WANG Jingchun
,
TIAN Kangming
,
MIAO Jia
,
WANG Caizhe
,
JIN Peng
,
WANG Zhengxiang
. Enhanced intracellular trehalose accumulation improves stress tolerance andethanol yield of Escherichia coli[J]. Food and Fermentation Industries, 2019
, 45(21)
: 15
-21
.
DOI: 10.13995/j.cnki.11-1802/ts.020729
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