To separate and purify polysaccharides from Gelidium amansii, and to analyze the physicochemical properties and monosaccharide composition of the purified polysaccharide component, GAP1. Crude polysaccharide was isolated from Gelidium amansii by multi-enzymatic extraction and deproteinized by Sevage method. The polysaccharide was fractionated and further purified into homogeneity by ion exchange chromatography on DEAE-52 column and Sephadex G-200 gel column. Its total carbohydrates, protein, uronic acid and sulfate radical contents were analyzed, and the monosaccharide composition was analyzed by high performance liquid chromatography (HPLC) after 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. The results showed that the purified polysaccharide designated as GAP1 was obtained; the total sugar content in GAP1 was 92.56%, the uronic acid content was 44.53%, and the sulfate radical content in GAP1 was 6.92% without protein; GAP1 was composed of rhamnose, glucuronic acid, glucose, galactose, xylose and L-fucose in different ratios, the molar ratio of monosaccharide was 1.00:1.00:4.45:27.31:2.27:1.88. This study provides a theoretical foundation for the high-value utilization of Gelidium amansii polysaccharides.
PEI Ruonan
,
ZHAI Honglei
,
QI Bo
,
HAO Shuxian
,
HUANG Hui
,
YANG Xianqing
. Isolation, purification and monosaccharide composition analysis of polysaccharide from Gelidium amansii[J]. Food and Fermentation Industries, 2020
, 46(7)
: 57
-62
.
DOI: 10.13995/j.cnki.11-1802/ts.023023
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