An approach to produce agmatine from arginine using arginine decarboxylase was developed. An arginine decarboxylase was expressed in Escherichia coli and the condition for enzyme expression was optimized. The activity of the enzyme increased 2.23 times by removing the inhibitive components and increasing the amount of those that promote protein expression. The effect of coenzyme on the yield of agmatine in the transformation process was also optimized. In pilot-scale in 15 L fermenter, the yield of agmatine reached 279.21 g/L with conversion rate of 98%.
ZHANG Yanhui
,
JI Wuke
,
GAO Aicun
,
SUN Botong
,
YUAN Jianguo
. Production of agmatine from arginine by enzyme transformation[J]. Food and Fermentation Industries, 2019
, 45(24)
: 40
-44
.
DOI: 10.13995/j.cnki.11-1802/ts.021597
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