A strain Lip-55 producing thermostable lipase was isolated from the sediment of Shijiaquan hot spring. It was identified as Acinetobacter sp. According to its morphology, physiological and biochemical characteristics, and 16S rRNA sequence analysis. The growth conditions and enzymatic properties were studied. The results showed that it could grow at 55 ℃ and the optimum growth temperature was 37 ℃. The lipase had maximum activity at 55 ℃ and t pH 5.0. Zn2+, Ca2+ and Mg2+ had a certain inhibitory effect on the enzyme activity. Mn2+, K+, Fe2+ and Cu2+ promoted the activity, of which Fe2+ was the most obvious.
CHEN Guiyuan
,
LIU Linbo
,
SANG Peng
,
YANG Liquan
. Isolation and identification of a thermostable lipase-producing strain and its enzymatic properties[J]. Food and Fermentation Industries, 2019
, 45(24)
: 52
-57
.
DOI: 10.13995/j.cnki.11-1802/ts.019562
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