This study is to exploit the biochemical properties of β-lactamase producing strains isolated from Shanghai Lingang area. The β-lactamase-producing strain was isolated by combination of antibiotic and the iodometry (qualitative) screening. Using morphology characterization, physiological and biochemical tests and 16S rRNA sequencing analysis, the strain was identified as Bacillus cereus B03. The enzymatic properties test results indicated that the optimum reaction conditions are at 40 ℃, and pH 7.0. The enzyme stored in the solution had good stability within range of 4- 20 ℃ and pH 5.0-7.5. The ion Mg2+, Fe2+ and Mn2+ had different degree of activation on the enzyme and Mg2+ showed the strongest effect. Meanwhile, the ion Cu2+, Al3+ and Fe3+ had different degree of inhibitory effects and Al3+ showed the strongest effect. No obvious effects of K+, Ca2+ and Zn2+ on the enzyme activity were found. After adding 50 g/L NaCl or 100 g/L glycerol to the aseptic crude enzyme solution, the enzyme activity remained 93% for 30 days storage.
CHEN Cheng
,
NING Xibin
. Screening, identification and analysis of β-lactamase-producing strains[J]. Food and Fermentation Industries, 2019
, 45(24)
: 77
-83
.
DOI: 10.13995/j.cnki.11-1802/ts.021904
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