In this research, an alkaline β-mannanase-producing strain was obtained and studied for potential industrial application. An alkaline bacterium with good enzyme-producing properties was isolated from natural soil. The strain was further identified as Bacillus cohnii using morphological and molecular biological analysis. After optimization using single-factor method and orthogonal experimental design, the amount of enzyme produced using shaking flask fermentation for 36 h was 345 U/L, which was 4.3 times of that before optimization. The optimum condition of the crude β-mannanase extract was determined to be at 55 ℃ and pH of 9.5. The results laid a foundation for scale-up fermentation of alkaline β-mannanase and provided a good strain for cloning and heterologous expression of alkaline β-mannanase encoding gene.
TANG Wenjing
,
LI Song
,
MA Zhongbao
,
YANG Qian
,
TANG Bin
. Screening, identification and optimization of fermentation conditions for alkaline β-mannanase-producing strain[J]. Food and Fermentation Industries, 2019
, 45(23)
: 50
-54
.
DOI: 10.13995/j.cnki.11-1802/ts.021511
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