Trehalose is a natural disaccharide synthesized by living organisms with stress tolerance and protection. It plays an important role in food processing and storage, as well as in microbial physiology. In this study, a rapid method for quantifying trehalose was developed using a selective trehalase which was obtained previously. The detection limit was 0.019mg/mL and its quantification limit was 0.1 mg/mL. The linear range of detection was between 0.1 mg/mL and 150.0 mg/mL. The product recovery rate was 98.80%-101.33%. This new method is simple, rapid, with high specificity and reproducible. It has potential applications in quantifying trehalose during strain development and raw material nutrient analysis.
MIAO Jia
,
WANG Caizhe
,
NIU Dandan
,
Nokuthula Peace MCHUNU
,
TIAN Kangming
,
Suren SINGH
,
Kugenthiren PERMAUL
,
WANG Zhengxiang
. A novel method for rapid determination of trehalose and its preliminary application[J]. Food and Fermentation Industries, 2019
, 45(23)
: 227
-231
.
DOI: 10.13995/j.cnki.11-1802/ts.022211
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