A novel method for rapid determination of trehalose and its preliminary application

  • MIAO Jia ,
  • WANG Caizhe ,
  • NIU Dandan ,
  • Nokuthula Peace MCHUNU ,
  • TIAN Kangming ,
  • Suren SINGH ,
  • Kugenthiren PERMAUL ,
  • WANG Zhengxiang
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  • 1 (Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology,Tianjin University of Science and Technology, Tianjin 300457, China)
    2 (College of Chemical Engineering and Materials Science, Tianjin University of Science andTechnology, Tianjin 300457, China)
    3 (Biotechnology Platform, Agricultural Research Council, Pretoria 0001, South Africa)
    4 (Department of Biotechnology & Food Technology, Durban University of Technology, Durban 4001, South Africa)

Received date: 2019-09-09

  Online published: 2020-02-11

Abstract

Trehalose is a natural disaccharide synthesized by living organisms with stress tolerance and protection. It plays an important role in food processing and storage, as well as in microbial physiology. In this study, a rapid method for quantifying trehalose was developed using a selective trehalase which was obtained previously. The detection limit was 0.019mg/mL and its quantification limit was 0.1 mg/mL. The linear range of detection was between 0.1 mg/mL and 150.0 mg/mL. The product recovery rate was 98.80%-101.33%. This new method is simple, rapid, with high specificity and reproducible. It has potential applications in quantifying trehalose during strain development and raw material nutrient analysis.

Cite this article

MIAO Jia , WANG Caizhe , NIU Dandan , Nokuthula Peace MCHUNU , TIAN Kangming , Suren SINGH , Kugenthiren PERMAUL , WANG Zhengxiang . A novel method for rapid determination of trehalose and its preliminary application[J]. Food and Fermentation Industries, 2019 , 45(23) : 227 -231 . DOI: 10.13995/j.cnki.11-1802/ts.022211

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