Flavor esters are important flavor components in food, beverage and personal care products. A novel lipase (CutA) with flavor ester synthesis activity was identified from Aspergillus niger by molecular cloning, heterologous expression and biochemical characterization. CutA was revealed that the condition of the maximal activity was 30℃ and pH 7.0. With synthetic substrate pNPA, its Km and Vmax were 19.50 mmol/L and 63.30 mmol/(mL·h), respectively. CutA was able to catalyze the synthesis of butyl butyrate, ethyl caprylate and ethyl lactate by esterification of corresponding acids and alcohols with conversion efficiencies of 17.2%, 4.7% and 1.8 % respectively under 30 ℃ in the organic solvent system. The results indicated a possible application of CutA for flavor improvement/modification of certain fermented foods.
CONG Shanzi
,
CHENG Lei
,
TIAN Kangming
,
LI Mengdi
,
LU Fuping
,
WANG Zhengxiang
. Aspergillus niger lipase CutA having flavor ester synthesis activity[J]. Food and Fermentation Industries, 2019
, 45(22)
: 1
-5
.
DOI: 10.13995/j.cnki.11-1802/ts.021313
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