The aim of this study was to investigate the tenderization effects and the changes of actomyosin content of lamb by sodium bicarbonate combined with ultrasound method. The lamb was treated with deionized water (W), sodium bicarbonate (S), deionized water with ultrasound (W+U) and sodium bicarbonate with ultrasound (S+U), with non-treatment as control group (C). The effects were characterized by the indexes of pH, cooking loss, texture, shear force, holding water capacity, tissue section and surficial microstructure, as well as the degree of actomyosin dissociation. Compared with the control, all indexes of group W+U, and S+U improved significantly. The lamb treated with S+U had the largest pH value, optimal texture parameters, obvious myofibril fragmentation and loose protein structure, contributing to the dissociation of actomyosin. Furthermore, its lowest cooking loss showed good water holding ability, and its shear value also decreased significantly. Sodium bicarbonate combined with ultrasound exhibited perfect tenderization effect, providing scientific evidence for further improving the quality of meat.
[1] WARNER R D, MCDONNELL C K, BEKHIT A E D, et al. Systematic review of emerging and innovative technologies for meat tenderisation[J]. Meat Science, 2017, 132: 72-89.
[2] CHEMAT F, ROMBAUT N, SIGAIRE A G, et al. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications A Review[J]. Ultrasonics Sonochemistry, 2017, 34: 540-560.
[3] CHEMAT F, KHAN M K. Applications of ultrasound in food technology: Processing, preservation and extraction[J]. Ultrasonics Sonochemistry, 2010, 18(4): 813-835.
[4] ZOU Ye, ZHANG Kun, ZHANG Xinxiao, et al. Optimization of goose breast meat tenderness by rapid ultrasound treatment using response surface methodology and artificial neural network[J]. Animal Science Journal, 2018, 89(9): 1 339-1 347.
[5] MOHAN A, JAICO T, KERR W, et al. Functional properties of bicarbonates on physicochemical attributes of ground beef[J]. LWT - Food Science and Technology, 2016, 70: 333-341.
[6] 李楠,张艳芳,韩剑飞,等. 滚揉过程中添加碳酸氢钠对鸡胸肉品质的影响[J]. 肉类工业, 2015(2): 13-15.
[7] YANG Huijuan, ZHANG Wangang, LI Teng, et al. Effect of protein structure on water and fat distribution during meat gelling[J]. Food Chemistry, 2016, 204: 239-245.
[8] HOPKINS D L, LITTLEFIELD P J, THOMPSON J M. A research note on factors affecting the determination of myofibrillar fragmentation[J]. Meat Science, 2000, 56(1): 19-22.
[9] XUE Siwen, XU Xinglian, SHAN Huimin, et al. Effects of high-intensity ultrasound, high-pressure processing, and high-pressure homogenization on the physicochemical and functional properties of myofibrillar proteins[J]. Innovative Food Science & Emerging Technologies, 2018, 45: 354-360.
[10] OKITANI A, ICHINOSE N, ITOH J, et al. Liberation of actin from actomyosin in meats heated to 65℃[J]. Meat Science, 2009, 81(3): 446-450.
[11] 马晓冰,苏琳,林在琼,等. 不同品种肉羊肌肉的糖酵解潜力及其与肉品质的相关性[J]. 食品科学, 2015, 36(15): 1-4.
[12] 薛维利. 提高火腿、红肠和烤肉口感的新方法[J]. 肉类工业, 2001(9): 13.
[13] 张坤,吴海虹,邹烨,等. 超声波联合一磷酸腺苷(AMP)对鸡胸肉的嫩化效果[J]. 江苏农业学报, 2018, 34(6): 167-174.
[14] FERGUSON D M, GERRARD D E. Regulation of post-mortem glycolysis in ruminant muscle[J]. Animal Production Science, 2014, 54(4): 464-481.
[15] HUFF-LONERGAN E, MITSUHASHI T, BEEKMAN D D, et al. Proteolysis of specific muscle structural proteins by μ-calpain at low pH and temperature is similar to degradation in postmortem bovine muscle[J]. Journal of Animal Science, 1996, 74(5): 993-1 008.
[16] 黄明,赵莲,徐幸莲,等. 钙离子和钙激活酶外源抑制剂对牛肉钙激活酶活性和超微结构的影响[J]. 南京农业大学学报, 2004, 27(4): 101-104.
[17] KRAUSE R J, OCKERMAN H W, KROL B, et al. Influence of tumbling, tumbling time, trim and sodium tripolyphosphate on quality and yield of cured hams[J]. Journal of Food Science, 2010, 43(3): 853-855.
[18] 李楠,张艳芳,韩剑飞,等. 滚揉过程中添加碳酸氢钠对鸡胸肉品质的影响[J]. 肉类工业, 2015(2): 13-15.
[19] JAYASOORIYA S D, TORLEY P J, D'ARCY B R, et al. Effect of high power ultrasound and ageing on the physical properties of bovine semitendinosus and longissimus muscles[J]. Meat Science, 2007, 75(4): 628-639.
[20] GOT F, CULIOLI J, BERGE P, et al. Effects of high-intensity high-frequency ultrasound on ageing rate, ultrastructure and some physicochemical properties of beef[J]. Meat Science, 1999, 51(1): 35-42.
[21] 陈立娟,李欣,李铮,等. 蛋白质磷酸化调控羊肉肌原纤维蛋白的功能[J]. 中国农业科学, 2016, 49(7): 1 360-1 370.
[22] 朱秋劲,罗爱平,林国虎,等. 超声波和气调贮藏对冷却牛肉保鲜效果的影响[J]. 食品科学, 2006,27(1): 240-246.
[23] 史培磊,闵辉辉,李春保,等. 滚揉腌制前后鹅肉品质的变化[J]. 食品科学, 2011, 32(11): 88-92.
[24] D′ALESSANDRO A, MARROCCO C, RINALDUCCI S, et al. Chianina beef tenderness investigated through integrated omics[J]. Journal of Proteomics, 2012, 75(14): 4 381-4 398.
[25] ASGHAR A, SAMEJIMA K, YASUI T, et al. Functionality of muscle proteins in gelation mechanisms of structured meat products[J]. Critical Reviews in Food Technology, 1985, 22(1): 27-106.
[26] WANG E, XU Weiwei, KANG Ning, et al. Microstructural, protein denaturation and water holding properties of lamb under pulse vacuum brining[J]. Meat Science, 2016, 113: 132-138.
[27] SIRÓ I, VÉN C S, BALLA C, et al. Application of an ultrasonic assisted curing technique for improving the diffusion of sodium chloride in porcine meat[J]. Journal of Food Engineering, 2009, 91(2): 353-362.
[28] PETIGNY L, PÉRINO-ISSARTIER S, WAJSMAN J, et al. Batch and continuous ultrasound assisted extraction of boldo leaves (Peumus boldusÉ Mol.)[J]. International Journal of Molecular Sciences, 2013, 14(3): 5 750-5 764.
[29] ERTBJERG P, HENCKEL P, KARLSSON A, et al. Combined effect of epinephrine and exercise on calpain/calpastatin and cathepsin B and L activity in porcine longissimus muscle[J]. Journal of Animal Science, 1999, 77(9): 2 428-2 436.
[30] 邓少颖,王道营,张牧焓,等. 鸭胸肌肉加热过程中肌动球蛋白解离研究[J]. 食品科学, 2015, 36(1): 1-5.