Sucrose-6-acetate is a key intermediate for sucralose preparation. Establishing an accurate and rapid analytical method for the determination of sucrose-6-acetate is essential for the quality control in sucralose production. Sucrose-6-acetate was detected by high performance liquid chromatography-evaporative light scattering (HPLC-ELSD). The analytical column was ZORBAX SB-Aq column with methanol/water (5∶90, V/V) as the mobile phase at a flow rate of 0.8 mL/min. The evaporative light drift tube temperature was 85 ℃ and the nitrogen flow rate was 1.5 L/min. The method was verified by internal standard method and external standard method for the detection of sucrose-6-acetate. The linear range of both methods was 1.10-11.03 mg/mL, with the linear correlation coefficient of above 0.99. The RSD value of the external standard method was less than 3.0%, and that of the internal standard method was less than 2.0%. In addition, the detection limit and quantification limit of the internal standard method were lower than the external standard method, which were 31.04 mg/mL and 103.46 mg/mL, respectively. Both methods could detect sucrose-6-acetate, and the internal standard method was slightly better than the external standard method. The method established in this study can be used for the determination of sucrose-6-acetate, which provides a reference for the detection and analysis of sucrose esters.
YANG Xinyi
,
GUO Hui
,
CHEN Yunfei
,
QIAN Junqing
,
ZHUANG Yong
. Determination of sucrose-6-acetate by high performance liquid chromatography[J]. Food and Fermentation Industries, 2019
, 45(22)
: 240
-245
.
DOI: 10.13995/j.cnki.11-1802/ts.021109
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